Recombinant Anti-TADA3L antibody [EPR13864] (ab181984)
![Flow Cytometry - Anti-TADA3L antibody [EPR13864] (ab181984)](https://www.abcam.com/ps/products/181/ab181984/Images/ab181984-212835-anti-tada3l-antibody-epr13864-flow-cytometry.jpg "Flow Cytometry - Anti-TADA3L antibody [EPR13864] (ab181984)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13864] to TADA3L
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TADA3L antibody [EPR13864]
See all TADA3L primary antibodies -
Description
Rabbit monoclonal [EPR13864] to TADA3L -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TADA3L aa 300-400. The exact sequence is proprietary.
Database link: O75528 -
Positive control
- HeLa, Jurkat and 293 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13864 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab181984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 49 kDa. | |
| Flow Cyt | 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
TADA3L functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TADA3L is also known as a coactivator for p53/TP53-dependent transcriptional activation. The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 10474 Human
- Entrez Gene: 101206 Mouse
- Entrez Gene: 362414 Rat
- Omim: 602945 Human
- SwissProt: O75528 Human
- SwissProt: Q8R0L9 Mouse
- SwissProt: Q4V8F5 Rat
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Alternative names
- ADA3 antibody
- ADA3 homolog antibody
- ADA3-like protein antibody
see all
Images
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Flow Cytometry - Anti-TADA3L antibody [EPR13864] (ab181984)
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling TADA3L with ab181984 at 1/90 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Western blot - Anti-TADA3L antibody [EPR13864] (ab181984)](https://www.abcam.com/ps/products/181/ab181984/Images/ab181984-212833-anti-tada3l-antibody-epr13864-western-blot.jpg)
Western blot - Anti-TADA3L antibody [EPR13864] (ab181984)All lanes : Anti-TADA3L antibody [EPR13864] (ab181984) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa -
![Anti-TADA3L antibody [EPR13864] (ab181984)](https://www.abcam.com/ps/products/181/ab181984/Images/ab181984-3-benefits-of-recombinant-antibodies.png)
Anti-TADA3L antibody [EPR13864] (ab181984)
Datasheets and documents
Certificate of Compliance
References (1)
ab181984 has been referenced in 1 publication.
- Zhang S et al. Overexpression of Alteration/Deficiency in Activation 3 correlates with poor prognosis in non-small cell lung cancer. Pathol Res Pract N/A:152408 (2019). PubMed: 30954347
