Recombinant Anti-TADA3L antibody [EPR13864] (ab181984)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TADA3L antibody [EPR13864]
See all TADA3L primary antibodies -
Description
Rabbit monoclonal [EPR13864] to TADA3L -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TADA3L aa 300-400. The exact sequence is proprietary.
Database link: O75528 -
Positive control
- HeLa, Jurkat and 293 cell lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13864 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab181984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 49 kDa. | |
| Flow Cyt | 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
TADA3L functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TADA3L is also known as a coactivator for p53/TP53-dependent transcriptional activation. The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 10474 Human
- Entrez Gene: 101206 Mouse
- Entrez Gene: 362414 Rat
- Omim: 602945 Human
- SwissProt: O75528 Human
- SwissProt: Q8R0L9 Mouse
- SwissProt: Q4V8F5 Rat
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Alternative names
- ADA3 antibody
- ADA3 homolog antibody
- ADA3-like protein antibody
see all
Images
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![Flow Cytometry - Anti-TADA3L antibody [EPR13864] (ab181984)](https://www.abcam.com/ps/products/181/ab181984/Images/ab181984-212835-anti-tada3l-antibody-epr13864-flow-cytometry.jpg)
Flow Cytometry - Anti-TADA3L antibody [EPR13864] (ab181984)Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling TADA3L with ab181984 at 1/90 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Western blot - Anti-TADA3L antibody [EPR13864] (ab181984)](https://www.abcam.com/ps/products/181/ab181984/Images/ab181984-212833-anti-tada3l-antibody-epr13864-western-blot.jpg)
Western blot - Anti-TADA3L antibody [EPR13864] (ab181984)All lanes : Anti-TADA3L antibody [EPR13864] (ab181984) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa
Datasheets and documents
References
This product has been referenced in:
- Zhang S et al. Overexpression of Alteration/Deficiency in Activation 3 correlates with poor prognosis in non-small cell lung cancer. Pathol Res Pract N/A:152408 (2019). Read more (PubMed: 30954347) »
