Key features and details
- Rabbit polyclonal to TAF1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Hamster, Human
- Isotype: IgG
Product nameAnti-TAF1 antibody
See all TAF1 primary antibodies
DescriptionRabbit polyclonal to TAF1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Human
- HeLa whole cell extract; BHK and HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab188427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|WB||1/1000. Predicted molecular weight: 213 kDa.|
RelevanceTAF1 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.
- BA2R antibody
- CCG 1 antibody
- CCG1 antibody
Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.
Anti-TAF1 antibody (ab188427) at 1/1000 dilution + HeLa whole cell extract at 10 µg
Predicted band size: 213 kDa
IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.
Panel a): Stained with ab188427 without immunizing peptide.
Panel b): Stained with ab188427 with immunizing peptide.
Panel c): Panel a) co-stained with Hoechst 33258.
Panel d): Panel b) co-stained with Hoechst 33258.
Panel e): Phase-contrast image of panels a) and c).
Panel f): Phase-contrast image of panels b) and d).
ab188427 has been referenced in 1 publication.
- Sekiguchi T et al. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol 11:3317-25 (1991). WB, ICC/IF . PubMed: 2038334