Product nameAnti-TAF1 antibody
See all TAF1 primary antibodies
DescriptionRabbit polyclonal to TAF1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human TAF1 aa 1800-1850. The exact sequence is proprietary. NP_620278.1
Database link: P21675
- IP: HEK-293T whole cell lysate. WB: HEK-293T, Jurkat and HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264327 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 213 kDa.|
RelevanceTAF1 functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Compared to higher eukaryotes TAF1, yeast TAF1 lacks the C-terminal bromodomains and C-terminal kinase activity. The TFIID interacting proteins BDF1 and BDF2 may substitute for these domains.
- BA2R antibody
- CCG 1 antibody
- CCG1 antibody
All lanes : Anti-TAF1 antibody (ab264327) at 0.1 µg/ml
Lane 1 : HEK-293T whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 213 kDa
Exposure time: 30 seconds
TAF1 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab264327 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264327 at 1 µg/ml.
Lane 1: ab264327 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264327 has not yet been referenced specifically in any publications.