Overview

  • Product name

  • Description

    Rabbit polyclonal to TAF11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment (aa1-176) corresponding to the N-terminal domain of TAF11 protein from budding yeast.

  • Positive control

    • Extract of budding yeast

Properties

Applications

Our Abpromise guarantee covers the use of ab64066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function

    Core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
  • Sequence similarities

    Belongs to the TAF11 family.
  • Domain

    TBP and TAFII18 bind to distinct domains of TAFII28.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

    • Alternative names

      • MGC:15243 antibody
      • p30 beta antibody
      • PRO2134 antibody
      • TAF antibody
      • TAF(II)28 antibody
      • TAF11 antibody
      • TAF11 RNA polymerase II TATA box binding protein (TBP) associated factor 28 kD antibody
      • TAF11_HUMAN antibody
      • TAF2I antibody
      • TAFII 28 antibody
      • TAFII-28 antibody
      • TAFII28 antibody
      • TFIID subunit p30 beta antibody
      • TFIID subunit p30-beta antibody
      • Transcription initiation factor TFIID 28 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 11 antibody
      see all

    Images

    • Anti-TAF11 antibody (ab64066) at 1/5000 dilution + Extract of budding yeast.

      Predicted band size: 40 kDa
      Observed band size: 45 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 28 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

    References

    ab64066 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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