Overview

  • Product name

    Anti-TAF12 antibody
  • Description

    Rabbit polyclonal to TAF12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Recombinant fragment within Human TAF12 (internal sequence). The exact sequence is proprietary.
    Database link: Q16514

  • Positive control

    • WB: HEK-293T, A431, HepG2 and HeLa whole cell extract; HeLa nuclear extract. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IP 1/100 - 1/500.

Target

  • Function

    TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the TAF12 family.
    Contains 1 histone-fold domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • TAF12 antibody
    • TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa antibody
    • TAF12_HUMAN antibody
    • TAF15 antibody
    • TAF2J antibody
    • TAFII 20/TAFII 15 antibody
    • TAFII-20/TAFII-15 antibody
    • TAFII20 antibody
    • TAFII20/TAFII15 antibody
    • Transcription initiation factor TFIID 20/15 kDa subunits antibody
    • Transcription initiation factor TFIID subunit 12 antibody
    see all

Images

  • All lanes : Anti-TAF12 antibody (ab229487) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 5 : HeLa nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

  • TAF12 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab229487. Western blot was performed from the immunoprecipitate using ab229487.

    Lane 1: Control IgG IP in HEK-293T whole cell extract.

    Lane 2: ab229487 IP in HEK-293T whole cell extract.

References

ab229487 has not yet been referenced specifically in any publications.

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