Product nameAnti-TAF12 antibody
DescriptionRabbit polyclonal to TAF12
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
Recombinant fragment within Human TAF12 (internal sequence). The exact sequence is proprietary.
Database link: Q16514
- WB: HEK-293T, A431, HepG2 and HeLa whole cell extract; HeLa nuclear extract. IP: HEK-293T whole cell extract.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229487 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
|IP||1/100 - 1/500.|
FunctionTAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription.
Sequence similaritiesBelongs to the TAF12 family.
Contains 1 histone-fold domain.
- Information by UniProt
- TAF12 antibody
- TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa antibody
- TAF12_HUMAN antibody
All lanes : Anti-TAF12 antibody (ab229487) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 5 : HeLa nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
15% SDS-PAGE gel.
TAF12 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab229487. Western blot was performed from the immunoprecipitate using ab229487.
Lane 1: Control IgG IP in HEK-293T whole cell extract.
Lane 2: ab229487 IP in HEK-293T whole cell extract.
ab229487 has not yet been referenced specifically in any publications.