Anti-TAF15 antibody (ab272864)
Key features and details
- Rabbit polyclonal to TAF15
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TAF15 antibody
See all TAF15 primary antibodies -
Description
Rabbit polyclonal to TAF15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human TAF15. The exact sequence is proprietary. Center region.
Database link: Q92804 -
Positive control
- WB: Raji and NIH/3T3 whole cell lysate. IHC: Human breast carcinoma and CL1-0 xenograft tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). -
Tissue specificity
Ubiquitous. Observed in all fetal and adult tissues. -
Involvement in disease
Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. -
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsDimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8148 Human
- Entrez Gene: 70439 Mouse
- Omim: 601574 Human
- SwissProt: Q92804 Human
- Unigene: 402752 Human
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Alternative names
- 68 kDa TATA-binding protein-associated factor antibody
- Npl3 antibody
- RBP56 antibody
see all
Images
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Anti-TAF15 antibody (ab272864) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
7.5% SDS PAGE
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Paraffin-embedded human breast carcinoma tissue stained for TAF15 protein using ab272864 at a 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate stained for TAF15 using ab272864 at a 1/200 dilution in ICC/IF. Merged with a DNA probe.
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Paraffin-embedded CL1-0 xenograft tissue stained for TAF15 using ab272864 at a 1/100 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Anti-TAF15 antibody (ab272864) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272864 has not yet been referenced specifically in any publications.