Overview

  • Product name

    Anti-TAF15 antibody [EPR9196(B)]
    See all TAF15 primary antibodies
  • Description

    Rabbit monoclonal [EPR9196(B)] to TAF15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human TAF15 (UniProt ID: Q92804).

  • Positive control

    • HeLa, 293T and Jurkat cell lysates; Human kidney tissue; 293T cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
    • Tissue specificity

      Ubiquitous. Observed in all fetal and adult tissues.
    • Involvement in disease

      Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
    • Sequence similarities

      Belongs to the RRM TET family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications

      Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 68 kDa TATA-binding protein-associated factor antibody
      • Npl3 antibody
      • RBP56 antibody
      • RBP56_HUMAN antibody
      • RNA binding protein 56 antibody
      • RNA-binding protein 56 antibody
      • TAF antibody
      • TAF(II)68 antibody
      • TAF15 antibody
      • TAF15 RNA polymerase II TATA box binding protein (TBP) associated factor 68kDa antibody
      • TAF2N antibody
      • TAFII68 antibody
      • TATA-binding protein-associated factor 2N antibody
      see all

    Images

    • Immunohistochemical analysis of TAF15 in paraffin embedded Human kidney tissue stained with ab133760 at a 1/100 dilution.
    • All lanes : Anti-TAF15 antibody [EPR9196(B)] (ab133760) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 62 kDa

    • Immunofluorescent staining of TAF15 in 293T cells stained with ab133760 at a 1/250 dilution.
    • Immunohistochemical analysis using ab133760 showing positive staining in Colonic adenocarcinoma tissue.

    • Immunohistochemical analysis using ab133760 showing positive staining in Normal uterus tissue.

    • Immunohistochemical analysis using ab133760 showing positive staining in Lung adenocarcinoma tissue.

    • Immunohistochemical analysis using ab133760 showing positive staining in Urinary bladder transitional carcinoma tissue.

    References

    This product has been referenced in:

    • Feng Y  et al. The onset of human ectopic pregnancy demonstrates a differential expression of miRNAs and their cognate targets in the Fallopian tube. Int J Clin Exp Pathol 7:64-79 (2014). IHC . Read more (PubMed: 24427327) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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