Overview

  • Product name
  • Description
    Rabbit polyclonal to TAF172
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region between residues 75 and 125 of human TAF172 (NP_003963.1).

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 207 kDa (predicted molecular weight: 207 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 8 HEAT repeats.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase BTAF1 antibody
    • ATP-dependent helicase BTAF1 antibody
    • B-TFIID transcription factor associated 170 kDa subunit antibody
    • B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae) antibody
    • B-TFIID transcription factor-associated 170 kDa subunit antibody
    • BTAF 1 RNA polymerase II antibody
    • BTAF1 antibody
    • BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa antibody
    • BTAF1_HUMAN antibody
    • MOT 1 antibody
    • MOT1 antibody
    • MOT1 Yeast homolog of antibody
    • TAF 172 antibody
    • TAF II 170 antibody
    • TAF(II)170 antibody
    • TAF-172 antibody
    • TAFII170 antibody
    • TATA binding protein associated factor 172 antibody
    • TATA-binding protein-associated factor 172 antibody
    • TBP associated factor 172 antibody
    • TBP-associated factor 172 antibody
    see all

Images

  • All lanes : Anti-TAF172 antibody (ab72285) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 207 kDa
    Observed band size: 207 kDa
    Additional bands at: 170 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

  • Detection of Human TAF172 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.

References

ab72285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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