Anti-Taf4 antibody (ab244306)
Key features and details
- Rabbit polyclonal to Taf4
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Taf4 antibody
See all Taf4 primary antibodies -
Description
Rabbit polyclonal to Taf4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Taf4 aa 456-597.
Sequence:VLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQIST VQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGA AQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCK
Database link: O00268 -
Positive control
- IHC-P: Human duodenum tissue. ICC/IF: U-2 OS cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab244306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6874 Human
- SwissProt: O00268 Human
- Unigene: 18857 Human
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Alternative names
- TAF2C antibody
- TAF2C1 antibody
- TAF4A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Taf4 antibody (ab244306)
Formalin-fixed, paraffin-embedded human duodenum tissue stained for Taf4 using ab244306 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Taf4 antibody (ab244306)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Taf4 (green) using ab244306 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab244306 has not yet been referenced specifically in any publications.
