Anti-Taf4 antibody (ab244306)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Taf4 antibody
See all Taf4 primary antibodies -
Description
Rabbit polyclonal to Taf4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Taf4 aa 456-597.
Sequence:VLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQIST VQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGA AQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCK
Database link: O00268 -
Positive control
- IHC-P: Human duodenum tissue. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab244306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6874 Human
- SwissProt: O00268 Human
- Unigene: 18857 Human
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Alternative names
- TAF2C antibody
- TAF2C1 antibody
- TAF4A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Taf4 antibody (ab244306)Formalin-fixed, paraffin-embedded human duodenum tissue stained for Taf4 using ab244306 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Taf4 antibody (ab244306)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Taf4 (green) using ab244306 at 4 µg/ml in ICC/IF.
Datasheets and documents
References
ab244306 has not yet been referenced specifically in any publications.
