Product nameAnti-Taf4 antibody
See all Taf4 primary antibodies
DescriptionRabbit polyclonal to Taf4
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant fragment which was over-expressed in E.coli using a plasmid carrying the N-terminal domain of Taf4 protein (1-200aa) of Saccharomyces cerevisiae and purified by chromatography.
- Extract of budding yeast cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab63910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).|
|ELISA||Use at an assay dependent dilution.|
RelevanceTaf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
- SwissProt: P50105 Saccharomyces cerevisiae
- TAF2C antibody
- TAF2C1 antibody
- TAF4A antibody
All lanes : Anti-Taf4 antibody (ab63910) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Extract of budding yeast
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Seizl M et al. Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization. Nucleic Acids Res : (2011). WB ; Saccharomyces cerevisiae . Read more (PubMed: 21498544) »