Overview

  • Product name

    Anti-TAF4B antibody - C-terminal
  • Description

    Rabbit polyclonal to TAF4B - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TAF4B aa 654-683 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    FLFIGALQKRILDIGKKHDITELNSDAVNL


    Database link: Q92750

  • Positive control

    • Human liver tissue; K562 cell lysate; K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab189428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 91 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Relevance

    TAF4B is a cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). This protein is preferentially expressed in ovarian granulosa cells and is highly expressed in B cells.
  • Cellular localization

    Nucleus. Cytoplasm. Export into the cytoplasm is mediated by a CRM1-independent nuclear export pathway and not by phosphorylation.
  • Database links

  • Alternative names

    • TAF(II)105 antibody
    • TAF2C2 antibody
    • TAF4b RNA polymerase II, TATA box binding protein (TBP) associated factor, 105kDa antibody
    • TAFII 105 antibody
    • TAFII105 antibody
    • TAFII105ALPHA antibody
    • TATA box binding protein (TBP) associated factor 4B antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase II, C2, 105kD antibody
    • Transcription initiation factor TFIID 105 kD subunit antibody
    • Transcription initiation factor TFIID 105 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 4B antibody
    see all

Images

  • Anti-TAF4B antibody - C-terminal (ab189428) at 1/1000 dilution + K562 cell lysate at 35 µg

    Predicted band size: 91 kDa

  • Flow cytometric analysis of K562 cells labeling TAF4B with ab189428 at 1/10 dilution (right histogram) compared to negative control cells (left histogram) using FITC-conjugated goat-anti-rabbit secondary antibodies.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling TAF4B with ab189428 at 5 μg/ml.

References

ab189428 has not yet been referenced specifically in any publications.

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