RecombinantRabMAb

Recombinant Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)

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Immunoprecipitation - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
  • Flow Cytometry - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
  • Western blot - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
  • Western blot - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
  • Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15433] to TAF5 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TAF5 antibody [EPR15433] - BSA and Azide free
    See all TAF5 primary antibodies

  • Description

    Rabbit monoclonal [EPR15433] to TAF5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TAF5 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15542

  • General notes

    Ab251201 is the carrier-free version of ab195236. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251201 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR15433

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab251201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    TAF5 is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It may play a role in helping to anchor Taf4 within the TFIID complex. It may be involved in transducing signals from various transcriptional regulators to the RNA polymerase II transcription machinery. TAF5 belongs to the TFIID complex which is composed of TATA binding protein (Tbp) and a number of TBP-associated factors (Tafs).

  • Cellular localization

    Nuclear

  • Database links

    see all

  • Alternative names

    • fc38g05 antibody
    • MGC123129 antibody
    • p85 antibody
    • TAF(II)100 antibody
    • TAF2D antibody
    • TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa antibody
    • TAFII 80 antibody
    • TAFII100 antibody
    • TATA box binding protein (TBP)-associated factor 2D antibody
    • TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD antibody
    • TBP-associated factor, RNA polymerase II, 100-KD antibody
    • Transcription initiation factor TFIID 100 kDa subunit antibody
    • Transcription initiation factor TFIID 85 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 5 antibody
    • wu:fc38g05 antibody
    • zgc:123129 antibody
    see all

Images

  • Immunoprecipitation - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    Immunoprecipitation - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    This data was developed using ab195236, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab195236 at 1/70 dilution (lane 1) or a Rabbit monoclonal IgG (lane 2).
    Western Blot performed using ab195236 at 1/1000 dilution (0.6µg/ml).
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
  • Flow Cytometry - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    Flow Cytometry - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    This data was developed using ab195236, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling TAF5 with ab195236 at 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
  • Western blot - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    Western blot - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    All lanes : Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab195236, the same antibody clone in a different buffer formulation.

    The observed MW is consistent with what has been described in the literature (PMID: 9045704).

  • Western blot - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    Western blot - Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    All lanes : Anti-TAF5 antibody [EPR15433] - C-terminal (ab195236) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab195236, the same antibody clone in a different buffer formulation.

    The observed MW is consistent with what has been described in the literature (PMID: 9045704).

  • Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)
    Anti-TAF5 antibody [EPR15433] - BSA and Azide free (ab251201)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251201? Please let us know so that we can cite the reference in this datasheet.

ab251201 has not yet been referenced specifically in any publications.

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