Key features and details
- Rabbit polyclonal to TAF6
- Suitable for: WB, ELISA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Product nameAnti-TAF6 antibody
See all TAF6 primary antibodies
DescriptionRabbit polyclonal to TAF6
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant protein, corresponding to N terminal amino acids 1-200 of TAF6 of budding yeast.
- Extract of budding yeast
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6
Preservative: 0.1% Sodium azide
Concentration information loading...
Purification notesab64060 was purified by chromatography
Our Abpromise guarantee covers the use of ab64060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 72 kDa).|
|ELISA||Use at an assay dependent dilution.|
RelevanceTAF6 is a subunit of transcription factor IID (TFIID). Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
- TAF2E antibody
- E 70/85kD antibody
- TAF 6 antibody
Anti-TAF6 antibody (ab64060) at 1/5000 dilution + Extract of budding yeast. at 0.2 mg/ml
Predicted band size: 72 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab64060 has not yet been referenced specifically in any publications.