Anti-TAF7 antibody [2C5] (ab57494)
Key features and details
- Mouse monoclonal [2C5] to TAF7
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TAF7 antibody [2C5] -
Description
Mouse monoclonal [2C5] to TAF7 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment: FIWNHGITLP LKNVRKRRFR KTAKKKYIES PDVEKEVKRL LSTDAEAVST RWEIIAEDET KEAENQGLDI SSPGMSGHRQ GHDSLEHDEL REIFN, corresponding to amino acids 130-224 of Human TAF7
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General notes
This product was changed from ascites to tissue culture supernatant on 12th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2C5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab57494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the TAF7 family. -
Domain
The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6879 Human
- Omim: 600573 Human
- SwissProt: Q15545 Human
- Unigene: 438838 Human
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Alternative names
- RNA polymerase II TBP-associated factor subunit F antibody
- TAF 7 antibody
- TAF(II)55 antibody
see all
Images
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TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
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TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.
This image was generated using the ascites version of the product.
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Lane 2 : Anti-TAF7 antibody [2C5] (ab57494) at 1/1250 dilution
Lane 3 : Anti-TAF7 antibody [2C5] (ab57494) at 1/2500 dilution
Lane 4 : Anti-TAF7 antibody [2C5] (ab57494) at 1/5000 dilution
Lane 5 : Anti-TAF7 antibody [2C5] (ab57494) at 1/10000 dilution
All lanes : whole cell lysate prepared from SW780 badder cancer cells
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG conjugated to HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutesLane 1: All Blue Biorad.Gel run under denaturing conditions with a 4-12% gradient.Blocking step performed using 5% milk for one hour at room temperature.Primary antibody incubated for 16 hours.
This image was generated using the ascites version of the product.
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ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab57494 has been referenced in 3 publications.
- Gegonne A et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol 32:1984-97 (2012). WB . PubMed: 22411629
- Devaiah BN & Singer DS Cross-talk Among RNA Polymerase II Kinases Modulates C-terminal Domain Phosphorylation. J Biol Chem 287:38755-66 (2012). WB . PubMed: 23027873
- Takahashi H et al. Proteomics Reveals a Physical and Functional Link between Hepatocyte Nuclear Factor 4{alpha} and Transcription Factor IID. J Biol Chem 284:32405-12 (2009). WB ; Human . PubMed: 19805548