Overview

  • Product name

    Anti-TAF7 antibody
  • Description

    Mouse monoclonal to TAF7
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment: FIWNHGITLP LKNVRKRRFR KTAKKKYIES PDVEKEVKRL LSTDAEAVST RWEIIAEDET KEAENQGLDI SSPGMSGHRQ GHDSLEHDEL REIFN, corresponding to amino acids 130-224 of Human TAF7

  • General notes

    This product was changed from ascites to tissue culture supernatant on 12th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab57494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the TAF7 family.
  • Domain

    The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • RNA polymerase II TBP-associated factor subunit F antibody
    • TAF 7 antibody
    • TAF(II)55 antibody
    • TAF2F antibody
    • TAF7 antibody
    • TAF7 RNA polymerase II antibody
    • TAF7 RNA polymerase II TATA box binding protein (TBP) associated factor 55 kDa antibody
    • TAF7_HUMAN antibody
    • TAFII 55 antibody
    • TAFII-55 antibody
    • TAFII55 antibody
    • TATA box binding protein (TBP) associated factor RNA polymerase II F 55kD antibody
    • TATA box binding protein associated factor 2F antibody
    • TBP associated factor F antibody
    • Transcription factor IID subunit TAFII55 antibody
    • Transcription initiation factor TFIID 55 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 7 antibody
    see all

Images

  • TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This image was generated using the ascites version of the product.

  • TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.

    This image was generated using the ascites version of the product.

  • Lane 2 : Anti-TAF7 antibody (ab57494) at 1/1250 dilution
    Lane 3 : Anti-TAF7 antibody (ab57494) at 1/2500 dilution
    Lane 4 : Anti-TAF7 antibody (ab57494) at 1/5000 dilution
    Lane 5 : Anti-TAF7 antibody (ab57494) at 1/10000 dilution

    All lanes : whole cell lysate prepared from SW780 badder cancer cells

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG conjugated to HRP

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes


    Lane 1: All Blue Biorad.Gel run under denaturing conditions with a 4-12% gradient.Blocking step performed using 5% milk for one hour at room temperature.Primary antibody incubated for 16 hours.

    This image was generated using the ascites version of the product.

    See Abreview

  • ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:

  • Gegonne A  et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol 32:1984-97 (2012). WB . Read more (PubMed: 22411629) »
  • Devaiah BN & Singer DS Cross-talk Among RNA Polymerase II Kinases Modulates C-terminal Domain Phosphorylation. J Biol Chem 287:38755-66 (2012). WB . Read more (PubMed: 23027873) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bladder cancer)
Specification
bladder cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TEG
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Karin Birkenkamp-Demtroeder

Verified customer

Submitted May 14 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (SW780 bladder cancer)
Loading amount
25 µg
Specification
SW780 bladder cancer
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Karin Birkenkamp-Demtroeder

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up