Product nameAnti-TAF7 antibody
DescriptionMouse monoclonal to TAF7
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab57494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.|
|IHC-P||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionFunctions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Sequence similaritiesBelongs to the TAF7 family.
DomainThe [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
- Information by UniProt
- RNA polymerase II TBP-associated factor subunit F antibody
- TAF 7 antibody
- TAF(II)55 antibody
Overlay histogram showing HeLa cells stained with ab57494 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57494, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
TAF7 antibody (ab57494) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lymph node.
TAF7 antibody (ab57494) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
Lane 2 : Anti-TAF7 antibody (ab57494) at 1/1250 dilution
Lane 3 : Anti-TAF7 antibody (ab57494) at 1/2500 dilution
Lane 4 : Anti-TAF7 antibody (ab57494) at 1/5000 dilution
Lane 5 : Anti-TAF7 antibody (ab57494) at 1/10000 dilution
All lanes : whole cell lysate prepared from SW780 badder cancer cells
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG conjugated to HRP
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Lane 1: All Blue Biorad.Gel run under denaturing conditions with a 4-12% gradient.Blocking step performed using 5% milk for one hour at room temperature.Primary antibody incubated for 16 hours.
ab57494 staining TAF7 in human bladder cancer tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TEG. Samples were then blocked with 1% serum for 1 hour at room temperature followed by incubation with the primary antibody at a 1/3500 dilution for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody.
This product has been referenced in:
- Gegonne A et al. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Mol Cell Biol 32:1984-97 (2012). WB . Read more (PubMed: 22411629) »
- Devaiah BN & Singer DS Cross-talk Among RNA Polymerase II Kinases Modulates C-terminal Domain Phosphorylation. J Biol Chem 287:38755-66 (2012). WB . Read more (PubMed: 23027873) »