Key features and details
- Rabbit polyclonal to TAF8/TBN
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TAF8/TBN antibody
DescriptionRabbit polyclonal to TAF8/TBN
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
- HEK lysate; Human placenta tissue; U-251 MG cells.
This product was previously labelled as TAF8
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204894 in the following tested applications.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 34 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.
Sequence similaritiesBelongs to the TAF8 family.
Contains 1 histone-fold domain.
DomainEctopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition.
Cellular localizationNucleus. Cytoplasm. Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.
- Information by UniProt
- FLJ32821 antibody
- hTAFII43 antibody
- Protein taube nuss antibody
All lanes : Anti-TAF8/TBN antibody (ab204894) at 1/100 dilution
Lane 1 : Negative Control
Lane 2 : HEK293 lysate
Predicted band size: 34 kDa
Immunofluorescent analysis of U-251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling TAF8/TBN with ab204894 at 4 µg/ml (green).
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TAF8/TBN with ab204894 at 1/50 dilution.
ab204894 has not yet been referenced specifically in any publications.