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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription TFIID
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Anti-TAF9 antibody (ab191702)

  • Datasheet
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Western blot - Anti-TAF9 antibody (ab191702)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)

Key features and details

  • Rabbit polyclonal to TAF9
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-TAF9 antibody
    See all TAF9 primary antibodies
  • Description

    Rabbit polyclonal to TAF9
  • Host species

    Rabbit
  • Specificity

    Multiple isoforms of TAF9 are known to exist. This antibody is predicted to not cross-react with other TAF family members.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TAF9 (internal sequence). The exact sequence is proprietary. 17 amino acid peptide
    Database link: Q16594

  • Positive control

    • HepG2 cell lysate, mouse liver tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • TFIID
    • Epigenetics and Nuclear Signaling
    • Transcription
    • RNA polymerase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HepG2 whole cell lysate (ab166833)
    • Mouse liver tissue lysate - total protein (ab29301)

Applications

Our Abpromise guarantee covers the use of ab191702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Relevance

    TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 6880 Human
    • Entrez Gene: 108143 Mouse
    • Entrez Gene: 373541 Rat
    • Omim: 600822 Human
    • SwissProt: Q16594 Human
    • SwissProt: Q8VI33 Mouse
    • SwissProt: Q5BKE0 Rat
    • Unigene: 653163 Human
    • Unigene: 301148 Mouse
    • Unigene: 8139 Rat
    see all
  • Alternative names

    • TAF9 RNA polymerase II, TATA box-binding protein-associated factor, 32kDa antibody
    • MGC:1603 antibody
    • MGC:3647 antibody
    • MGC:5067 antibody
    • RNA polymerase II TBP-associated factor subunit G antibody
    • STAF31/32 antibody
    • TAF17 antibody
    • TAF2G antibody
    • TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa antibody
    • TAFII31 antibody
    • TAFII32 antibody
    • TAFIID32 antibody
    • TATA box-binding protein-associated factor 2G antibody
    • TBP associated factor 17 kDa antibody
    • TBP associated factor 9 antibody
    • TBP associated factor, RNA polymerase II, 32-KD antibody
    • transcription initiation factor TFIID 31 kD subunit antibody
    • transcription initiation factor TFIID 31 kDa subunit antibody
    • transcription initiation factor TFIID 32 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 9 antibody
    see all

Images

  • Western blot - Anti-TAF9 antibody (ab191702)
    Western blot - Anti-TAF9 antibody (ab191702)
    Lane 1 : Anti-TAF9 antibody (ab191702) at 1 µg/ml
    Lane 2 : Anti-TAF9 antibody (ab191702) at 2 µg/ml

    All lanes : HepG2 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)

    Immunohistochemical analysis of mouse liver tissue labeling TAF9 using ab191702 at 5 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)

    Immunohistochemical staining analysis of mouse liver tissue labeling TAF9 using ab191702 at 20 μg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab191702? Please let us know so that we can cite the reference in this datasheet.

    ab191702 has not yet been referenced specifically in any publications.

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