Anti-TAF9 antibody (ab191702)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TAF9 antibody
See all TAF9 primary antibodies -
Description
Rabbit polyclonal to TAF9 -
Host species
Rabbit -
Specificity
Multiple isoforms of TAF9 are known to exist. This antibody is predicted to not cross-react with other TAF family members. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TAF9 (internal sequence). The exact sequence is proprietary. 17 amino acid peptide
Database link: Q16594 -
Positive control
- HepG2 cell lysate, mouse liver tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab191702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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Relevance
TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6880 Human
- Entrez Gene: 108143 Mouse
- Entrez Gene: 373541 Rat
- Omim: 600822 Human
- SwissProt: Q16594 Human
- SwissProt: Q8VI33 Mouse
- SwissProt: Q5BKE0 Rat
- Unigene: 653163 Human
see all -
Alternative names
- TAF9 RNA polymerase II, TATA box-binding protein-associated factor, 32kDa antibody
- MGC:1603 antibody
- MGC:3647 antibody
see all
Images
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Western blot - Anti-TAF9 antibody (ab191702)Lane 1 : Anti-TAF9 antibody (ab191702) at 1 µg/ml
Lane 2 : Anti-TAF9 antibody (ab191702) at 2 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)Immunohistochemical analysis of mouse liver tissue labeling TAF9 using ab191702 at 5 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAF9 antibody (ab191702)Immunohistochemical staining analysis of mouse liver tissue labeling TAF9 using ab191702 at 20 μg/ml.
Datasheets and documents
References
ab191702 has not yet been referenced specifically in any publications.
