Key features and details
- Rabbit polyclonal to TAF9
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TAF9 antibody
See all TAF9 primary antibodies
DescriptionRabbit polyclonal to TAF9
SpecificityMultiple isoforms of TAF9 are known to exist. This antibody is predicted to not cross-react with other TAF family members.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TAF9 (internal sequence). The exact sequence is proprietary. 17 amino acid peptide
Database link: Q16594
- HepG2 cell lysate, mouse liver tissue
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
RelevanceTAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
- TAF9 RNA polymerase II, TATA box-binding protein-associated factor, 32kDa antibody
- MGC:1603 antibody
- MGC:3647 antibody
Lane 1 : Anti-TAF9 antibody (ab191702) at 1 µg/ml
Lane 2 : Anti-TAF9 antibody (ab191702) at 2 µg/ml
All lanes : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 29 kDa
Immunohistochemical analysis of mouse liver tissue labeling TAF9 using ab191702 at 5 μg/ml.
Immunohistochemical staining analysis of mouse liver tissue labeling TAF9 using ab191702 at 20 μg/ml.
ab191702 has not yet been referenced specifically in any publications.