• Product name

    Anti-TAF9 antibody [EPR12075(B)] (HRP)
    See all TAF9 primary antibodies
  • Description

    Rabbit monoclonal [EPR12075(B)] to TAF9 (HRP)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human TAF9 aa 50-150. The exact sequence is proprietary.
    Database link: Q16594

  • Positive control

    • WB: K562 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab209310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).


  • Relevance

    TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
  • Cellular localization

  • Database links

  • Alternative names

    • TAF9 RNA polymerase II, TATA box-binding protein-associated factor, 32kDa antibody
    • MGC:1603 antibody
    • MGC:3647 antibody
    • MGC:5067 antibody
    • RNA polymerase II TBP-associated factor subunit G antibody
    • STAF31/32 antibody
    • TAF17 antibody
    • TAF2G antibody
    • TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa antibody
    • TAFII31 antibody
    • TAFII32 antibody
    • TAFIID32 antibody
    • TATA box-binding protein-associated factor 2G antibody
    • TBP associated factor 17 kDa antibody
    • TBP associated factor 9 antibody
    • TBP associated factor, RNA polymerase II, 32-KD antibody
    • transcription initiation factor TFIID 31 kD subunit antibody
    • transcription initiation factor TFIID 31 kDa subunit antibody
    • transcription initiation factor TFIID 32 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 9 antibody
    see all


  • Anti-TAF9 antibody [EPR12075(B)] (HRP) (ab209310) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 3 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209310 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.


ab209310 has not yet been referenced specifically in any publications.

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