Anti-tag-72 antibody [B72.3 + CC49] (ab199002)
Key features and details
- Mouse monoclonal [B72.3 + CC49] to tag-72
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Rat, Cow, Dog, Human
- Isotype: IgG1
Overview
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Product name
Anti-tag-72 antibody [B72.3 + CC49] -
Description
Mouse monoclonal [B72.3 + CC49] to tag-72 -
Host species
Mouse -
Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Cow, Dog, Human
Predicted to work with: Caenorhabditis elegans -
Immunogen
Other Immunogen Type corresponding to Human tag-72. Membrane-enriched fraction of a human breast carcinoma liver metastasis [B72.3]; Purified TAG-72 protein [CC49].
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Positive control
- IHC-P: Human prostate and ovarian carcinoma tissue.
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General notes
Both antibodies (B72.3 + CC49) are mouse IgG1 kappa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
B72.3 + CC49 -
Isotype
IgG1 -
Light chain type
kappa
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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Flow Cyt |
Use 1-2µg for 106 cells.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use 1-2µg for 106 cells. |
Target
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Database links
- Entrez Gene: 182875 Caenorhabditis elegans
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Alternative names
- CA 72.4 antibody
- Tumor associated glycoprotein 72 antibody
Images
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab199002 has been referenced in 2 publications.
- Gaiteiro C et al. Glycoproteogenomics characterizes the CD44 splicing code associated with bladder cancer invasion. Theranostics 12:3150-3177 (2022). PubMed: 35547758
- Peixoto A et al. Glycoproteomics identifies HOMER3 as a potentially targetable biomarker triggered by hypoxia and glucose deprivation in bladder cancer. J Exp Clin Cancer Res 40:191 (2021). PubMed: 34108014