Key features and details
- Mouse monoclonal [B72.3 + CC49] to tag-72
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF, IHC-Fr
- Reacts with: Rat, Cow, Dog, Human
- Isotype: IgG1
Product nameAnti-tag-72 antibody [B72.3 + CC49]
DescriptionMouse monoclonal [B72.3 + CC49] to tag-72
SpecificityRecognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas.
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Rat, Cow, Dog, Human
Predicted to work with: Caenorhabditis elegans
Recombinant full length protein corresponding to Caenorhabditis elegans tag-72 aa 1-380.
MSSSEVASHYNKVLQVGIEGRKESRIFFMRNMNNWVKSQLINDAKQRVND NGVNNPRVLDLACGKGGDLKKWDIAGAKDVVMADVADVSIQQAEERYKQM FGYKKNNIFTVQFIVADCTKENLEDRIENKDPFDLVSCQFALHYSFVDEA SARIFLKNAVGMLKPGGVFIGTLPDADRIVWSMRNGENGQFANEVCKITY ENVEELAEGKVPLFGAKFHFSLDEQVNCPEFLAYFPLVKHLLEELDMELL FVHNFAEAINKWLEPGRRLLESMTGLETYPNEKLSGKSDDEYLEAKAKLD AFPEDERIKTMGTLSKSEWEAICMYLVFGFRKKKSEAEKTEEEPATTKPV AESESEQKEVTESEEKEDQEDCEHQEAQTN
Database link: Q9XVS1
- Jurkat cells, Breast or lung carcinoma.
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Both antibodies (B72.3 + CC49) are mouse IgG1 kappa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Clone numberB72.3 + CC49
Light chain typekappa
Our Abpromise guarantee covers the use of ab199002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 220 kDa.|
|IP||Use at 2-4 µg/mg of lysate.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 106 cells.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
- CA 72.4 antibody
- Tumor associated glycoprotein 72 antibody
ab199002 has not yet been referenced specifically in any publications.