IHC protocols

Are you new to immunohistochemistry or immunofluorescence? Perhaps you wish to try a new immunostaining method and don't know where to start? Our detailed IHC and ICC/IF protocols cover all aspects of specimen preparation for ICC/IF and IHC, whether you are staining paraffin-embedded, frozen or free-floating sections or whole tissue mounts. You will also find step-by-step tips for mouse on mouse staining, BrdU staining, and non-antibody-based techniques for neuronal staining such as Bielschowsky's silver stain. Once you've obtained the information you need to be an IHC and ICC/IF expert be sure to check out our extensive ranges of reagents such as our primary antibodies, validated in immunohistochemistry and immunofluorescence

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  • Protocols

IHC tissue processing protocol

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ICC/IF protocol

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BrdU staining protocol

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In situ hybridization protocol

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IHC deparaffinization protocol

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IHC FAQs

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IHC antigen retrieval protocol

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IHC fixation protocol

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IHC permeabilization protocol

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