Secondary antibodies

We have grouped our secondary antibody resources (protocols, products, events and webinars) to make it easier for you to choose your product of interest. We have over 2500 high quality purified secondary antibodies, unconjugated and conjugated forms, validated in major applications such as western blot, IHC, ICC and flow cytometry. Discover how Abcam can support your research.

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Articles
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Products
Protocols
Videos

Immunoprecipitation protocol

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western-blot

Fluorescent Western Blotting

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Advanced Immunoprecipitation

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Primary antibodies

Organelle markers and dyes

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secondary-antibodies

Find out how to use IRDye® secondary antibodies, with abcam Fluorescent WB protocol.

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ICC/IF

Principles and Practice of Confocal Microscopy in Life Sciences

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ICC

IHC/ICC Staining Techniques Using Single and Multiple Labels

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Secondary Antibodies

Secondary antibodies optimized for co-IP western blotting

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Secondary antibodies

Detect low-abundance proteins with biotinylated antibodies

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Secondary Antibodies

Higher sensitivity,low background in your IHC using HRP-polymer secondaries

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Secondary Antibodies

Secondary F(ab) & F(ab')2 antibody fragments conjugated to HRP, AP, and Alexa Fluor® dyes

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Secondary antibodies

Pre-adsorbed secondary antibodies - minimize non-specific binding and high background staining

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Secondary antibodies

Our guide to secondary antibody selection

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secondary-antibodies

Hints and tips for fluorescent western blotting

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secondary-antibodies

IRDye® conjugated secondary antibodies

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Western blot

Procedure for detection of phosphorylated proteins in western blot

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Secondary Antibodies

HRP or AP conjugated secondary antibodies optimized for western blotting

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Secondary antibodies

HRP Secondary Antibodies

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secondary-antibodies

Double and triple immunostaining tips.

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Reagents

B cells: Basic immunophenotyping

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Secondary antibodies

Learn about cell imaging techniques through our protocol and webinar series

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secondary-antibodies/alexa-conjugated-secondaries

Fluorochrome chart – a complete guide

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Reagents

T cells: basic immunophenotyping

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Antibody guide

Choosing a primary antibody or secondary antibody

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Western blot

Western blot FAQ

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Primary Antibodies

Comprehensive guide to Human CD antigens

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Secondary antibodies

Flow cytometry – Experimental design and sample preparation

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Secondary antibodies

Fluorescence guide

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Secondary Antibodies

Direct vs indirect immunofluorescence

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Secondary Antibodies

Conjugate selection guide for secondary antibodies

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Secondary antibodies

Validated Alexa Fluor secondary antibodies for guaranteed performance

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Flow Cyt

Thoroughly validated directly conjugated antibodies

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Flow Cyt

Color Compensation in Flow Cytometry

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Flow Cyt

Understanding fluorophores

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Primary antibodies

Hints and tips to optimize your ICC and immunofluorescence experiments

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ICC/IF

An Introduction to ICC Principles and Troubleshooting

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Secondary antibodies

The definitive guide for choosing the correct HRP secondary antibody for your immunohistochemistry (IHC) experiments

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Secondary antibodies

Why choosing the right secondary antibody matters?

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Secondary antibodies

Multiplexing in fluorescent western blotting

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Secondary Antibodies

Alexa Fluor® conjugated secondaries ideal for multicolor analysis

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Secondary antibodies

Alexa Fluor® 488-conjugated antibodies | Abcam

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secondary-antibodies

Advantages Of immunoglobulin F(ab) and F(ab')2 fragments

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Primary antibodies

Mounting media and sera for imaging

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Fluorescent dyes

A guide to fluorochromes poster

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Secondary Antibodies

Multi-color cell imaging - a useful guide to help choose the right products for your experiments

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Secondary antibodies

Secondaries you can trust

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IHC

Enzyme conjugated secondary antibodies and fragments - ideal for ELISA and IHC

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Secondary Antibodies

Secondary antibodies conjugated to gold nanoparticles for use in electron microscopy

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Support

Protocols and troubleshooting tips, to help get the best from our antibodies

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Secondary Antibodies

Alexa Fluor conjugated secondary antibodies - FAQs

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Primary antibodies

Imaging reagents tested for quality

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Secondary Antibodies

Secondary antibodies for veterinary research

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Secondary Antibodies

Fluorescent western blot Alexa Fluor® secondaries

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Primary antibodies

Flow cytometric analysis of extracellular vesicles

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Neuroscience

IHC protocols

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Secondary antibodies

DyLight® Fluorochrome conjugated secondary antibodies

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cytopainter

Cell tracking staining kit

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cytopainter

Lysosomal staining reagents

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Cytopainter

Fluorescent staining of viable cells

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cytopainter

Golgi apparatus staining kits

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Cytopainter

Stain actin filaments with phalloidin conjugates

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Secondary antibodies

Unconjugated Secondary Antibodies

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Secondary Antibodies

Alexa Fluor 680 conjugated secondaries optimized for fluorescent-WB

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