Secondary antibodies

We have grouped our secondary antibody resources (protocols, products, events and webinars) to make it easier for you to choose your product of interest. We have over 2500 high quality purified secondary antibodies, unconjugated and conjugated forms, validated in major applications such as western blot, IHC, ICC and flow cytometry. Discover how Abcam can support your research.

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Thoroughly validated directly conjugated antibodies

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T cells: basic immunophenotyping

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To make sure you get the faster results in immunostaining with your primary antibody, get the tested secondary antibody too.

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Immunoprecipitation protocol

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Hints and tips for fluorescent western blotting

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Secondary antibodies conjugated to gold nanoparticles for use in electron microscopy

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Hints and tips to optimize your ICC and immunofluorescence experiments

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IHC/ICC Staining Techniques Using Single and Multiple Labels

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Fluorescent Western Blotting

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Advanced Immunoprecipitation

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Principles and Practice of Confocal Microscopy in Life Sciences

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Western blot FAQ

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Direct vs indirect immunofluorescence

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Advantages Of immunoglobulin F(ab) and F(ab')2 fragments

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The definitive guide for choosing the correct HRP secondary antibody for your immunohistochemistry (IHC) experiments

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Fluorescence guide

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IRDye® conjugated secondary antibodies

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Learn about cell imaging techniques through our protocol and webinar series

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Alexa Fluor® conjugated secondaries ideal for multicolor analysis

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Mounting media and sera for imaging

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Organelle markers and dyes

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Protocols and troubleshooting tips, to help get the best from our antibodies

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HRP Secondary Antibodies

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Flow cytometric analysis of extracellular vesicles

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A guide to fluorochromes poster

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Alexa Fluor 680 conjugated secondaries optimized for fluorescent-WB

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Detect low-abundance proteins with biotinylated antibodies

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Conjugate selection guide for secondary antibodies

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Secondaries you can trust

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Double and triple immunostaining tips.

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Fluorochrome chart – a complete guide

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Anti-mouse IgG for IP (HRP) (ab131368)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)

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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)

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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)

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Goat Anti-Human IgG Fc (HRP) (ab97225)

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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)

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Procedure for detection of phosphorylated proteins in western blot

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Our guide to secondary antibody selection

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Validated Alexa Fluor secondary antibodies for guaranteed performance

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Goat Anti-Mouse IgG H&L (FITC) (ab6785)

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Find out how to use IRDye® secondary antibodies, with abcam Fluorescent WB protocol.

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Secondary antibodies optimized for co-IP western blotting

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Pre-adsorbed secondary antibodies - minimize non-specific binding and high background staining

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HRP or AP conjugated secondary antibodies optimized for western blotting

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B cells: Basic immunophenotyping

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Choosing a primary antibody or secondary antibody

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Comprehensive guide to Human CD antigens

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Flow cytometry – Experimental design and sample preparation

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Understanding fluorophores

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Why choosing the right secondary antibody matters?

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Multiplexing in fluorescent western blotting

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Alexa Fluor® 488-conjugated antibodies | Abcam

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Enzyme conjugated secondary antibodies and fragments - ideal for ELISA and IHC

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Alexa Fluor conjugated secondary antibodies - FAQs

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Fluorescent western blot Alexa Fluor® secondaries

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IHC protocols

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DyLight® Fluorochrome conjugated secondary antibodies

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Cell tracking staining kit

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Lysosomal staining reagents

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Fluorescent staining of viable cells

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Golgi apparatus staining kits

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Stain actin filaments with phalloidin conjugates

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Unconjugated Secondary Antibodies

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