Secondary antibodies
We have grouped our secondary antibody resources (protocols, products, events and webinars) to make it easier for you to choose your product of interest. We have over 2500 high quality purified secondary antibodies, unconjugated and conjugated forms, validated in major applications such as western blot, IHC, ICC and flow cytometry. Discover how Abcam can support your research.
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Hints and tips to optimize your ICC and immunofluorescence experiments
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Organelle markers and dyes
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Alexa Fluor® conjugated secondaries ideal for multicolor analysis
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Thoroughly validated directly conjugated antibodies
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Fluorochrome chart – a complete guide
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Fluorescent Western Blotting
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Anti-mouse IgG for IP (HRP) (ab131368)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
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Secondary antibodies for veterinary research
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Higher sensitivity,low background in your IHC using HRP-polymer secondaries
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (ab150115)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-Human IgG4 pFc’ [HP6023] (Biotin) (ab99818)
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Goat Anti-Human IgG Fc (HRP) (ab97225)
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
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Color Compensation in Flow Cytometry
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Procedure for detection of phosphorylated proteins in western blot
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Mounting media and sera for imaging
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Our guide to secondary antibody selection
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Validated Alexa Fluor secondary antibodies for guaranteed performance
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Fluorescence guide
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Advanced Immunoprecipitation
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Immunoprecipitation protocol
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Goat Anti-Mouse IgG H&L (FITC) (ab6785)
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Principles and Practice of Confocal Microscopy in Life Sciences
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IHC/ICC Staining Techniques Using Single and Multiple Labels
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Secondary antibodies optimized for co-IP western blotting
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Detect low-abundance proteins with biotinylated antibodies
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Hints and tips for fluorescent western blotting
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IRDye® conjugated secondary antibodies
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HRP or AP conjugated secondary antibodies optimized for western blotting
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HRP Secondary Antibodies
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Double and triple immunostaining tips.
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B cells: Basic immunophenotyping
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Learn about cell imaging techniques through our protocol and webinar series
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T cells: basic immunophenotyping
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Choosing a primary antibody or secondary antibody
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Western blot FAQ
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Comprehensive guide to Human CD antigens
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Flow cytometry – Experimental design and sample preparation
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Direct vs indirect immunofluorescence
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Conjugate selection guide for secondary antibodies
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Understanding fluorophores
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An Introduction to ICC Principles and Troubleshooting
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Why choosing the right secondary antibody matters?
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Multiplexing in fluorescent western blotting
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Alexa Fluor® 488-conjugated antibodies | Abcam
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Advantages Of immunoglobulin F(ab) and F(ab')2 fragments
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A guide to fluorochromes poster
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Secondaries you can trust
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Protocols and troubleshooting tips, to help get the best from our antibodies
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Alexa Fluor conjugated secondary antibodies - FAQs
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Imaging reagents tested for quality
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Fluorescent western blot Alexa Fluor® secondaries
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Flow cytometric analysis of extracellular vesicles
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IHC protocols
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DyLight® Fluorochrome conjugated secondary antibodies
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Cell tracking staining kit
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Lysosomal staining reagents
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Fluorescent staining of viable cells
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Golgi apparatus staining kits
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Stain actin filaments with phalloidin conjugates
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Unconjugated Secondary Antibodies
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Alexa Fluor 680 conjugated secondaries optimized for fluorescent-WB
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