Recombinant Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5106] to TAG1/TAX1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TAG1/TAX1 antibody [EPR5106]
See all TAG1/TAX1 primary antibodies -
Description
Rabbit monoclonal [EPR5106] to TAG1/TAX1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human TAG1/TAX1 aa 1-100. The exact sequence is proprietary.
-
Positive control
- Fetal brain, Human cerebellum, U87-MG, and SH-SY5Y cell lysates, U87-MG cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.02 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5106 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 113 kDa).
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 113 kDa). |
Target
-
Function
May play a role in the initial growth and guidance of axons. May be involved in cell adhesion. -
Sequence similarities
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. Attached to the neuronal membrane by a GPI-anchor and is also released from neurons. - Information by UniProt
-
Database links
- Entrez Gene: 6900 Human
- Entrez Gene: 21367 Mouse
- Omim: 190197 Human
- SwissProt: Q02246 Human
- SwissProt: Q61330 Mouse
- Unigene: 519220 Human
- Unigene: 260861 Mouse
-
Alternative names
- Axonal glycoprotein TAG 1 antibody
- Axonal glycoprotein TAG-1 antibody
- Axonal glycoprotein TAG1 antibody
see all
Images
-
All lanes : Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) at 1/10000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : Human cerebellum lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometrical analysis of permeablized U87-MG cells using ab133498 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab133498 has been referenced in 3 publications.
- Kuwayama R et al. Establishment of mouse model of inherited PIGO deficiency and therapeutic potential of AAV-based gene therapy. Nat Commun 13:3107 (2022). PubMed: 35661110
- Fathi A et al. Discovery of Novel Cell Surface Markers for Purification of Embryonic Dopamine Progenitors for Transplantation in Parkinson's Disease Animal Models. Mol Cell Proteomics 17:1670-1684 (2018). PubMed: 29848781
- Zhen YB et al. Expression of TAG1/APP signaling pathway in the proliferation and differentiation of glioma stem cells. Oncol Lett 14:2137-2140 (2017). PubMed: 28789439