Product nameAnti-TAG1/TAX1 antibody [EPR5106]
See all TAG1/TAX1 primary antibodies
DescriptionRabbit monoclonal [EPR5106] to TAG1/TAX1
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TAG1/TAX1 aa 1-100. The exact sequence is proprietary.
- Fetal brain, Human cerebellum, U87-MG, and SH-SY5Y cell lysates, U87-MG cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as TAG1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.02 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 113 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionMay play a role in the initial growth and guidance of axons. May be involved in cell adhesion.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane. Attached to the neuronal membrane by a GPI-anchor and is also released from neurons.
- Information by UniProt
- Axonal glycoprotein TAG 1 antibody
- Axonal glycoprotein TAG-1 antibody
- Axonal glycoprotein TAG1 antibody
All lanes : Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : U87-MG lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Flow Cytometrical analysis of permeablized U87-MG cells using ab133498 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Equilibrium disassociation constant (KD)
Learn more about KD
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