Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5106] to TAG1/TAX1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-TAG1/TAX1 antibody [EPR5106]
See all TAG1/TAX1 primary antibodies
DescriptionRabbit monoclonal [EPR5106] to TAG1/TAX1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TAG1/TAX1 aa 1-100. The exact sequence is proprietary.
- Fetal brain, Human cerebellum, U87-MG, and SH-SY5Y cell lysates, U87-MG cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as TAG1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.02 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 113 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay play a role in the initial growth and guidance of axons. May be involved in cell adhesion.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane. Attached to the neuronal membrane by a GPI-anchor and is also released from neurons.
- Information by UniProt
- Axonal glycoprotein TAG 1 antibody
- Axonal glycoprotein TAG-1 antibody
- Axonal glycoprotein TAG1 antibody
All lanes : Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : U87-MG lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Flow Cytometrical analysis of permeablized U87-MG cells using ab133498 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133498 has been referenced in 1 publication.
- Zhen YB et al. Expression of TAG1/APP signaling pathway in the proliferation and differentiation of glioma stem cells. Oncol Lett 14:2137-2140 (2017). PubMed: 28789439