• Product name

    Anti-TAG72 antibody [B72.3]
  • Description

    Mouse monoclonal [B72.3] to TAG72
  • Host species

  • Specificity

    This antibody recognises an oncofetal antigen of >1,000kDa, identified as a Tumor-Associated Glycoprotein (TAG-72) with the properties of a mucin. Weak or no reactivity has been observed with most cell types of normal adult tissue excepting the secretory endometrium.
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat, Hamster, Human
  • Immunogen

    Membrane-enriched fraction of a human breast carcinoma liver metastasis

  • Positive control

    • Lung carcinoma or breast cancer.



Our Abpromise guarantee covers the use of ab691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Incubation for 30-60 minutes at room temperature is recommended.
IHC-Fr Use at an assay dependent concentration.


  • Relevance

    The majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for TAG72. About 60% of carcinoma patients express TAG72 in their sera. TAG72 is reportedly useful in distinguishing pulmonary adenocarcinomas from pleural mesotheliomas.
  • Cellular localization

  • Alternative names

    • CA 72.4 antibody
    • CA72.4 antibody
    • TAG 72 antibody
    • Tumor associated glycoprotein 72 antibody


This product has been referenced in:

  • Xu F  et al. Expression profile of mucin-associated sialyl-Tn antigen in Chinese patients with different colorectal lesions (adenomas, carcinomas). Int J Clin Exp Pathol 8:11549-54 (2015). Read more (PubMed: 26617889) »
  • van Niekerk CC  et al. Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detect Prev 21:247-57 (1997). Read more (PubMed: 9167042) »
See all 12 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for your phone call this week and for your patience while I looked into your questions. I have heard back from the production lab of ab691, and the IgG concentrations vary among the lots that we have in stock: 60ug/mL for lot # 958786 33.3ug/mL for lots # 958785, 958787, 975535, and 975468   The protein carrier in the concentrated antibody diluent is BSA and the preservative is sodium azide.  I hope this information is helpful, but please let me know if you have any further questions.

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Thank you for your reply. Both of these antibodies are tissue culture supernatant meaning that it is not possible to determine antibody concentration, since tissue culture supernatant contains proteins besides the antibody of interest. Supernatant is the fluid resulting from centrifugation of hybridomas in tissue culture that are secreting specific monoclonal antibodies. Supernatants therefore typically contain culture medium, 5-10% fetal calf serum, and other proteins. Antibody concentration is only relevant for purified antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. There's no concentration information available for this antibody as it is provided in an unpurified form (tissue culture supernatant) and we cannot determine the concentration for unpurified products. To use the antibody in IHC-P, it is recommended to dilute the antibody 1/50-1/100. Please contact us again if you have any additional questions.

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Thank you for your enquiry. The concentration of the lots that we currently have in stock is 66 ug/ml. If you have any additional questions, please contact us again.

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Thank you for your enquiry. We are very sorry to hear that you are having some difficulty with this product. We would like to confirm that the two batches you are having are different, however we have not received any technical enquiry which would indicate that there is some batch-related problem. We would like to emphasize that this product has not been characterised for ELISA application only for IHC staining. We hope this information will be useful.

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