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Anti-TAGAP antibody [EPR15593]
Rabbit monoclonal [EPR15593] to TAGAP
Recombinant fragment within Human TAGAP aa 250-400. The exact sequence is proprietary.
Database link: Q8N103
A549, Jurkat and HepG2 cell lysates; Jurkat cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/40.
1/1000 - 1/2000. Detects a band of approximately 61, 81 kDa (predicted molecular weight: 81 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Information by UniProt
Flow Cytometry - Anti-TAGAP antibody [EPR15593] (ab187664)
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TAGAP with ab187664 at 1/160 dilution (pink) compared to a Rabbit monoclonal IgG Isotype control (green). Goat anti rabbit IgG (FITC) was used as the secondary antibody at 1/150 dilution.
Western blot - Anti-TAGAP antibody [EPR15593] (ab187664)
All lanes :
Anti-TAGAP antibody [EPR15593] (ab187664) at 1/1000 dilution
Lane 1 :
Jurkat cell lysate
Lane 2 :
HepG2 cell lysate
Lane 3 :
A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size:
Observed band size:
61,81 kDa (
why is the actual band size different from the predicted?
Based on sequence analysis, ab187664 recognizes three isoforms with the predicted MWs of 81kDa, 61kDa and 57kDa, respectively.
Immunoprecipitation - Anti-TAGAP antibody [EPR15593] (ab187664)
Western blot analysis of TAGAP in A549 cell lysate immunoprecipitated with ab187664 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"