Overview

  • Product name
    Anti-TAGLN antibody [EPR11995(B)]
    See all TAGLN primary antibodies
  • Description
    Rabbit monoclonal [EPR11995(B)] to TAGLN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TAGLN aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01995

  • Positive control
    • Human bladder, stomach and uterus lysates; Human smooth muscle, smooth vessels and stomach tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11995(B)
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab170902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance
      Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • 22 kDa actin-binding protein antibody
      • Protein WS3-10 antibody
      • SM22 antibody
      • SM22-alpha antibody
      • Smooth muscle protein 22-alpha antibody
      • Transgelin antibody
      • WS3-10 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling TAGLN using ab170902 at a 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human smooth vessels tissue, labeling TAGLN using ab170902 at a 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling TAGLN using ab170902 at a 1/100 dilution.

    • All lanes : Anti-TAGLN antibody [EPR11995(B)] (ab170902) at 1/1000 dilution

      Lane 1 : Human bladder lysate
      Lane 2 : Human stomach lysate
      Lane 3 : Human uterus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa

    References

    This product has been referenced in:
    • Yoshida T  et al. Smooth Muscle-Selective Nuclear Factor-?B Inhibition Reduces Phosphate-Induced Arterial Medial Calcification in Mice With Chronic Kidney Disease. J Am Heart Assoc 6:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 29146611) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170902.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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