Key features and details
- Rabbit polyclonal to TAGLN2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TAGLN2 antibody
See all TAGLN2 primary antibodies
DescriptionRabbit polyclonal to TAGLN2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
antigen, corresponding to the N-terminal region amino acids 4-136 of the Human TAGLN2 protein (P37802).
- IHC-P: Human bone marrow tissue. WB: NIH/3T3, NBT-II, A431 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
Sequence similaritiesBelongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (actin filaments). Recommended concentration of ab121146 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 2 : Anti-TAGLN2 antibody (ab121146) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : A431 cells
Immunohistochemical staining of Human bone marrow with ab121146 at 1/500 dilution.
All lanes : Anti-TAGLN2 antibody (ab121146) at 0.4 µg/ml
Lane 1 : NIH/3T3 cells
Lane 2 : NBT-II cells
ab121146 has been referenced in 3 publications.
- Zhao F et al. MicroRNA-133b suppresses bladder cancer malignancy by targeting TAGLN2-mediated cell cycle. J Cell Physiol 234:4910-4923 (2019). PubMed: 30317571
- Zhao F et al. Identification of hub miRNA biomarkers for bladder cancer by weighted gene coexpression network analysis. Onco Targets Ther 10:5551-5559 (2017). PubMed: 29200870
- Xiao G et al. MiR-133b Regulates the Expression of the Actin Protein TAGLN2 during Oocyte Growth and Maturation: A Potential Target for Infertility Therapy. PLoS One 9:e100751 (2014). WB . PubMed: 24959893