Key features and details
- Rabbit polyclonal to TAGLN/Transgelin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TAGLN/Transgelin antibody
See all TAGLN/Transgelin primary antibodies
DescriptionRabbit polyclonal to TAGLN/Transgelin
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
Recombinant fragment corresponding to Human TAGLN/Transgelin aa 1-201.
MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRL GFQVWLKNGVILSKLVNSLYPDGSKPVKVPENPPSMVFKQMEQVAQFLKA AEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPN WFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGMTGYGRPRQII S
Database link: Q01995
- HeLa cells; HepG2 whole cell lysate
This product was previously labelled as TAGLN
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 23 kDa.|
FunctionActin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Sequence similaritiesBelongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain.
- Information by UniProt
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
Anti-TAGLN/Transgelin antibody (ab137453) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS PAGE
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling SM22 alpha with ab137453 at a 1/200 dilution. The image in the lower panel is merged with a DNA probe.
ab137453 has been referenced in 2 publications.
- Zheng X et al. The phenotype of vascular smooth muscle cells co-cultured with endothelial cells is modulated by PDGFR-ß/IQGAP1 signaling in LPS-induced intravascular injury. Int J Med Sci 16:1149-1156 (2019). PubMed: 31523178
- Klose R et al. Loss of the serine protease HTRA1 impairs smooth muscle cells maturation. Sci Rep 9:18224 (2019). PubMed: 31796853