Anti-TAGLN/Transgelin antibody (ab14106)
Key features and details
- Rabbit polyclonal to TAGLN/Transgelin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-TAGLN/Transgelin antibody
See all TAGLN/Transgelin primary antibodies -
Description
Rabbit polyclonal to TAGLN/Transgelin -
Host species
Rabbit -
Specificity
The immunogen used to generate this TAGLN/Transgelin antibody (ab14106) shares 75% homology with family members TAGLN2 and TAGLN3. Cross-reactivity with these proteins has not been confirmed experimentally.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Mouse TAGLN/Transgelin aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab16067) -
Positive control
- WB: Human primary smooth muscle cells; HeLa whole cell lysate; Mouse colon tissue lysate; Rat colon tissue lysate. ICC: HeLa cells; mouse muscle cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (8) |
Use a concentration of 1 - 5 µg/ml.
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| WB | (12) |
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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| Notes |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. -
Sequence similarities
Belongs to the calponin family.
Contains 1 calponin-like repeat.
Contains 1 CH (calponin-homology) domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6876 Human
- Entrez Gene: 21345 Mouse
- Entrez Gene: 25123 Rat
- SwissProt: Q01995 Human
- SwissProt: P37804 Mouse
- SwissProt: P31232 Rat
- Unigene: 410977 Human
- Unigene: 283283 Mouse
see all -
Alternative names
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
see all
Images
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Western blot - Anti-TAGLN/Transgelin antibody (ab14106)This image is a courtesy of Anonymous AbreviewAll lanes : Anti-TAGLN/Transgelin antibody (ab14106) at 1/1000 dilution
All lanes : Lysates prepared from human primary smooth muscle cells
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated bovine polyclonal to rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 2 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (ab14106)ab14106 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol for 5 minutes, permeabilized in 0.1% PBS-Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14106 at 1 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.
DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1 hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (ab14106)This image is courtesy of an anonymous Abreview.ab14106 staining SM22 alpha in mouse muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000.
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Western blot - Anti-TAGLN/Transgelin antibody (ab14106)All lanes : Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa nuclear extract
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaThe band was completed abolished by peptide blocking with ab16067 (immunizing peptide) - not shown.
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Western blot - Anti-TAGLN/Transgelin antibody (ab14106)All lanes : Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051)
Lane 2 : Mouse colon tissue lysate
Lane 3 : Rat colon tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 115 kDa, 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab14106 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-TAGLN/Transgelin antibody (ab14106)Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/ml +Recombinant Human TAGLN/Transgelin protein (ab101469) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (321)
ab14106 has been referenced in 321 publications.
- Furmanik M et al. Endoplasmic Reticulum Stress Mediates Vascular Smooth Muscle Cell Calcification via Increased Release of Grp78 (Glucose-Regulated Protein, 78 kDa)-Loaded Extracellular Vesicles. Arterioscler Thromb Vasc Biol 41:898-914 (2021). PubMed: 33297752
- Su T et al. Single-Cell Transcriptomics Reveals Zone-Specific Alterations of Liver Sinusoidal Endothelial Cells in Cirrhosis. Cell Mol Gastroenterol Hepatol 11:1139-1161 (2021). PubMed: 33340713
- Ren M et al. LncRNA H19 regulates smooth muscle cell functions and participates in the development of aortic dissection through sponging miR-193b-3p. Biosci Rep 41:N/A (2021). PubMed: 33403385
- Gatti F et al. Nuclear IL-33 restrains the early conversion of fibroblasts to an extracellular matrix-secreting phenotype. Sci Rep 11:108 (2021). PubMed: 33420328
- Zhang Q et al. MiR-30c-5p regulates adventitial progenitor cells differentiation to vascular smooth muscle cells through targeting OPG. Stem Cell Res Ther 12:67 (2021). PubMed: 33468212