Overview

  • Product name

    Anti-TAGLN/Transgelin antibody
    See all TAGLN/Transgelin primary antibodies
  • Description

    Rabbit polyclonal to TAGLN/Transgelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Recombinant fragment corresponding to Human TAGLN/Transgelin (internal sequence).
    Database link: Q01995

  • Positive control

    • Recombinant Human SM22 alpha protein (ab101469) can be used as a positive control in WB. HepG2 whole cell lysate, Mouse liver whole cell lysate, A-10 cells treated with MG132. HeLa cells. Ca922 xenograft.
  • General notes

     This product was previously labelled as TAGLN

     

Properties

Applications

Our Abpromise guarantee covers the use of ab155272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • 22 kDa actin-binding protein antibody
    • Protein WS3-10 antibody
    • SM22 antibody
    • SM22-alpha antibody
    • Smooth muscle protein 22-alpha antibody
    • Transgelin antibody
    • WS3-10 antibody
    see all

Images

  • Anti-TAGLN/Transgelin antibody (ab155272) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Anti-TAGLN/Transgelin antibody (ab155272) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • All lanes : Anti-TAGLN/Transgelin antibody (ab155272) at 1/5000 dilution

    Lane 1 : A-10 whole cell lysate
    Lane 2 : A-10 whole cell lysate treated with MG132

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 23 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling SM22 alpha with ab155272 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, labeling SM22 alpha with ab155272 at 1/100 dilution.

References

This product has been referenced in:

  • Lai YL  et al. Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells. Redox Biol 15:51-61 (2018). WB . Read more (PubMed: 29216542) »
  • Zhou H  et al. Elevated transgelin/TNS1 expression is a potential biomarker in human colorectal cancer. Oncotarget 9:1107-1113 (2018). Read more (PubMed: 29416680) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Human Cultured Cells (Human aortic SMCs)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Human aortic SMCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Simon Shen

Verified customer

Submitted Apr 09 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (aortic smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
aortic smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Mc Shen

Verified customer

Submitted May 30 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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