Recombinant Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-TAGLN/Transgelin antibody [EPR11995(B)]
See all TAGLN/Transgelin primary antibodies -
Description
Rabbit monoclonal [EPR11995(B)] to TAGLN/Transgelin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TAGLN/Transgelin aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q01995 -
Positive control
- Human bladder, stomach and uterus lysates; Human smooth muscle, smooth vessels and stomach tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as TAGLN
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11995(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 6876 Human
- SwissProt: Q01995 Human
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Alternative names
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)](https://www.abcam.com/ps/products/170/ab170902/Images/ab170902-172122-anti-tagln-antibody-epr11995b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling TAGLN/Transgelin using ab170902 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)](https://www.abcam.com/ps/products/170/ab170902/Images/ab170902-172121-anti-tagln-antibody-epr11995b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)Immunohistochemical analysis of paraffin-embedded Human smooth vessels tissue, labeling TAGLN/Transgelin using ab170902 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)](https://www.abcam.com/ps/products/170/ab170902/Images/ab170902-172120-anti-tagln-antibody-epr11995b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling TAGLN/Transgelin using ab170902 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)](https://www.abcam.com/ps/products/170/ab170902/Images/ab170902-172119-anti-tagln-antibody-epr11995b-western-blot.jpg)
Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)All lanes : Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902) at 1/1000 dilution
Lane 1 : Human bladder lysate
Lane 2 : Human stomach lysate
Lane 3 : Human uterus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Datasheets and documents
References
This product has been referenced in:
- Li F et al. Up- and downregulation of mature miR-1587 function by modulating its G-quadruplex structure and using small molecules. Int J Biol Macromol 121:127-134 (2019). Read more (PubMed: 30290263) »
- Yoshida T et al. Smooth Muscle-Selective Nuclear Factor-?B Inhibition Reduces Phosphate-Induced Arterial Medial Calcification in Mice With Chronic Kidney Disease. J Am Heart Assoc 6:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 29146611) »
