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RecombinantRabMAb

Recombinant Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
  • Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
  • Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11995(B)] to TAGLN/Transgelin
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-TAGLN/Transgelin antibody [EPR11995(B)]
    See all TAGLN/Transgelin primary antibodies
  • Description

    Rabbit monoclonal [EPR11995(B)] to TAGLN/Transgelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TAGLN/Transgelin aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01995

  • Positive control

    • Human bladder, stomach and uterus lysates; Human smooth muscle, smooth vessels and stomach tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as TAGLN

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11995(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Anti-TAGLN/Transgelin antibody [EPR11995(B)] - BSA and Azide free (ab249579)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TAGLN/Transgelin protein (ab101469)

Applications

Our Abpromise guarantee covers the use of ab170902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
    • Sequence similarities

      Belongs to the calponin family.
      Contains 1 calponin-like repeat.
      Contains 1 CH (calponin-homology) domain.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q01995 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6876 Human
      • SwissProt: Q01995 Human
      • Unigene: 410977 Human
      • Alternative names

        • 22 kDa actin-binding protein antibody
        • Protein WS3-10 antibody
        • SM22 antibody
        • SM22-alpha antibody
        • Smooth muscle protein 22-alpha antibody
        • TAGL_HUMAN antibody
        • TAGLN antibody
        • Transgelin antibody
        • WS3-10 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)

        Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling TAGLN/Transgelin using ab170902 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)

        Immunohistochemical analysis of paraffin-embedded Human smooth vessels tissue, labeling TAGLN/Transgelin using ab170902 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)

        Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling TAGLN/Transgelin using ab170902 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
        Western blot - Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
        All lanes : Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902) at 1/1000 dilution

        Lane 1 : Human bladder lysate
        Lane 2 : Human stomach lysate
        Lane 3 : Human uterus lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 23 kDa

      • Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)
        Anti-TAGLN/Transgelin antibody [EPR11995(B)] (ab170902)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab170902? Please let us know so that we can cite the reference in this datasheet.

      ab170902 has been referenced in 2 publications.

      • Li F  et al. Up- and downregulation of mature miR-1587 function by modulating its G-quadruplex structure and using small molecules. Int J Biol Macromol 121:127-134 (2019). PubMed: 30290263
      • Yoshida T  et al. Smooth Muscle-Selective Nuclear Factor-?B Inhibition Reduces Phosphate-Induced Arterial Medial Calcification in Mice With Chronic Kidney Disease. J Am Heart Assoc 6:N/A (2017). IHC-P ; Mouse . PubMed: 29146611

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab170902.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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