Overview

  • Product name

    Anti-TAGLN/Transgelin antibody [EPR21206]
    See all TAGLN/Transgelin primary antibodies
  • Description

    Human monoclonal [EPR21206] to TAGLN/Transgelin
  • Host species

    Human
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human normal colon tissue lysate , mouse normal bladder tissue lysate and normal colon tissue lysate gave positive results in western bot. IHC-P: FFPE human and mouse normal bladder tissue sections.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody.  
    Human monoclonal antibody.

     This product was previously labelled as TAGLN

     

Properties

Applications

Our Abpromise guarantee covers the use of ab213273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Relevance

    Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • 22 kDa actin-binding protein antibody
    • Protein WS3-10 antibody
    • SM22 antibody
    • SM22-alpha antibody
    • Smooth muscle protein 22-alpha antibody
    • Transgelin antibody
    • WS3-10 antibody
    see all

Images

  • All lanes : Anti-TAGLN/Transgelin antibody [EPR21206] (ab213273) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Human colon tissue lysate
    Lane 3 : Mouse skeletal muscle tissue lysate
    Lane 4 : Mouse Colon tissue lysate
    Lane 5 : Mouse Bladder Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Human IgG H&L (IRDye® 800CW) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Human IgG H&L (IRDye® 800CW) preabsorbed secondary at 1/10000 dilution for 1 hour. 

  • IHC image of SM22 alpha staining in a section of formalin-fixed paraffin-embedded normal mouse bladder performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213273, 5ug/ml, for 15 mins at room temperature.

    An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of SM22 alpha staining in a section of formalin-fixed paraffin-embedded normal human bladder* performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213273, 5ug/ml, for 15 mins at room temperature.

    An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab213273 has not yet been referenced specifically in any publications.

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