Product nameAnti-TAGLN/Transgelin antibody [EPR21215]
See all TAGLN/Transgelin primary antibodies
DescriptionRabbit monoclonal [EPR21215] to TAGLN/Transgelin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Human colon, Mouse colon and bladder tissue lysates gave a positive signal in western blot.
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
This product was previously labelled as TAGLN
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
Our Abpromise guarantee covers the use of ab227566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
RelevanceActin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
- 22 kDa actin-binding protein antibody
- Protein WS3-10 antibody
- SM22 antibody
All lanes : Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566) at 1 µg/ml
Lane 1 : Human Skeletal Muscle tissue lysate
Lane 2 : Human Colon tissue lysate
Lane 3 : Mouse Skeletal Muscle tissue lysate
Lane 4 : Mouse Colon tissue lysate
Lane 5 : Mouse Bladder tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 secondary at 1/10000 dilution for 1 hour.
ab227566 has not yet been referenced specifically in any publications.