Overview

  • Product name

    Anti-TAGLN/Transgelin antibody [EPR21215]
    See all TAGLN/Transgelin primary antibodies
  • Description

    Rabbit monoclonal [EPR21215] to TAGLN/Transgelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human colon, Mouse colon and bladder tissue lysates gave a positive signal in western blot.
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

     This product was previously labelled as TAGLN

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Relevance

    Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • 22 kDa actin-binding protein antibody
    • Protein WS3-10 antibody
    • SM22 antibody
    • SM22-alpha antibody
    • Smooth muscle protein 22-alpha antibody
    • Transgelin antibody
    • WS3-10 antibody
    see all

Images

  • All lanes : Anti-TAGLN/Transgelin antibody [EPR21215] (ab227566) at 1 µg/ml

    Lane 1 : Human Skeletal Muscle tissue lysate
    Lane 2 : Human Colon tissue lysate
    Lane 3 : Mouse Skeletal Muscle tissue lysate
    Lane 4 : Mouse Colon tissue lysate
    Lane 5 : Mouse Bladder tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 40 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213273 overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 secondary at 1/10000 dilution for 1 hour. 

References

ab227566 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227566.
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