Overview

  • Product name

  • Description

    Rabbit polyclonal to TAK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human TAK1. Derived from around the phosphorylation site of threonine 187 (H-M-T(p)-N-N).
    Database link: O43318

  • Positive control

    • Human breast carcinoma tissue; Jurkat cell extract, treated with heat shock, Jurkat cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab196955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.

Target

  • Function

    Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
    Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
  • Information by UniProt
  • Database links

  • Alternative names

    • M3K7_HUMAN antibody
    • MAP3K 7 antibody
    • Map3k7 antibody
    • MEKK7 antibody
    • Mitogen activated protein kinase kinase kinase 7 antibody
    • Mitogen-activated protein kinase kinase kinase 7 antibody
    • TAK1 antibody
    • TGF beta activated kinase 1 antibody
    • TGF-beta-activated kinase 1 antibody
    • TGF1a antibody
    • Transforming growth factor beta activated kinase 1 antibody
    • Transforming growth factor-beta-activated kinase 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TAK1 with ab196955 at 1/50 dilution. The image on the right is preincubated with blocking peptide.

  • All lanes : Anti-TAK1 antibody (ab196955) at 1/500 dilution

    Lane 1 : Jurkat cell extract, treated with heat shock
    Lane 2 : Jurkat cell extract, treated with heat shock with blocking peptide

    Predicted band size: 67 kDa

  • All lanes : Anti-TAK1 antibody (ab196955) at 1/500 dilution

    Lane 1 : Jurkat cell extract with blocking peptide
    Lane 2 : Jurkat cell extract

    Predicted band size: 67 kDa

References

ab196955 has not yet been referenced specifically in any publications.

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