Product nameAnti-TAK1 antibody
See all TAK1 primary antibodies
DescriptionRabbit polyclonal to TAK1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 14 amino acids near the amino terminus of TAK1 isoform (Human)(the amino acid sequence is considered to be commercially sensitive)
- Rat thymus cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Purification notesIon exchange chromatography purified IgG.
Our Abpromise guarantee covers the use of ab25879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa. The antibody will recognise all 4 isoforms of TAK1. The variation in the MW may also be caused by protein hydrophobicity, migrational speed or post-translational modifications.|
FunctionComponent of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsAssociation with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
- Information by UniProt
- M3K7_HUMAN antibody
- MAP3K 7 antibody
- Map3k7 antibody
Lane 1 : Anti-TAK1 antibody (ab25879) at 1 µg/ml
Lane 2 : Anti-TAK1 antibody (ab25879) at 2 µg/ml
Lane 3 : Anti-TAK1 antibody (ab25879) at 4 µg/ml
All lanes : Rat Thymus Tissue Lysate (ab4041)
Predicted band size: 67 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?