Key features and details
- Goat polyclonal to TAK1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TAK1 antibody
See all TAK1 primary antibodies
DescriptionGoat polyclonal to TAK1
SpecificityThis antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Orangutan
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab50431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 67 kDa).
A 1 hour primary incubation is recommended for this product. Approx 75kDa band observed in lysates of cell lines NIH/3T3 and A431.
FunctionComponent of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
modificationsAssociation with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
- Information by UniProt
- M3K7_HUMAN antibody
- MAP3K 7 antibody
- Map3k7 antibody
Lanes 1-2 : Anti-TAK1 antibody (ab50431) at 1 µg/ml
Lane 3 : Anti-TAK1 antibody (ab50431) at 2 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Daudi cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 67 kDa
Anti-TAK1 antibody (ab50431) at 0.1 µg/ml + Cell lysates prepared from NIH/3T3 cells at 35 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Primary antibody incubated for 1 hour.
ab50431 has not yet been referenced specifically in any publications.