Other product details
Host species: Donkey
Conjugation: Alexa Fluor® 680
lane 2 - transfected TAK1
-Shorter primary antibody incubations could be tried in addition to diluting the antibodies in the blocking buffer (optimal would be to try both milk and BSA).
-Exposure times could be varied too.
-To lower background, add 0.1 - 0.2% Tween-20 to the diluted antibody before incubation.
-Wash solutions should contain 0.1% Tween-20.
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Submitted Sep 17 2010