Overview

  • Product name
    Anti-TAK1 (phospho S439) antibody [EPR2863]
    See all TAK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2863] to TAK1 (phospho S439)
  • Host species
    Rabbit
  • Specificity
    ab109404 only detects TAK1 phosphorylated at Serine 439 in Human or TAK1 phosphorylated at Serine 412 in Mouse.
  • Tested applications
    Suitable for: Dot blot, WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho specific peptide against residues surrounding Serine 439 of Human TAK1.

  • Positive control
    • HeLa cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
IP 1/10 - 1/100.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
      Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
    • Information by UniProt
    • Database links
    • Alternative names
      • M3K7_HUMAN antibody
      • MAP3K 7 antibody
      • Map3k7 antibody
      • MEKK7 antibody
      • Mitogen activated protein kinase kinase kinase 7 antibody
      • Mitogen-activated protein kinase kinase kinase 7 antibody
      • TAK1 antibody
      • TGF beta activated kinase 1 antibody
      • TGF-beta-activated kinase 1 antibody
      • TGF1a antibody
      • Transforming growth factor beta activated kinase 1 antibody
      • Transforming growth factor-beta-activated kinase 1 antibody
      see all

    Images

    • All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1ß for 10 minutes whole cell lysate
      Lane 3 : HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1ß for 10 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 67 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Dilution and blocking buffer: 5% NFDM/TBST.

    • Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.

    • All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with transforming growth factor-beta (TGF-beta)

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 67 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Wang G  et al. Lycorine Suppresses Endplate-Chondrocyte Degeneration and Prevents Intervertebral Disc Degeneration by Inhibiting NF-?B Signalling Pathway. Cell Physiol Biochem 45:1252-1269 (2018). Read more (PubMed: 29448253) »
    • Liu Z  et al. Activation of TGF-ß activated kinase 1 promotes colon mucosal pathogenesis in inflammatory bowel disease. Physiol Rep 5:N/A (2017). Read more (PubMed: 28373409) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us and your interest in our products.

    Unfortunately, I can say that the anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) has been tried in IHC of paraffin embedded tissue sections, however it was found not to be suitable in this application. This information is stated under the "Applications" sectionof the datasheet.

    However, the anti-TAK1 (phospho T187) antibody (ab79583) has not been tested and may be suitable for this application. As we have not tried it ourselves we would not be able to guarantee this. As you have mentioned, you would be eligible for the testing discount with this antibody if you would be interested. This offer means that if you purchase ab79583 as normal, test the antibody with yourtissue samples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab79583 off any product). More information on this offer can be found here:

    www.abcam.com/collaborationdiscount

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab79583.

    Please be aware that this particular antibody is directed against the TAK1 protein phosphorylated on the tyrosine residue187. We have several other anitbodies raised against the totalTAK1 protein which can be accessed from the following link:

    https://www.abcam.com/index.html?t=86756&pt=1

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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