Recombinant Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
![Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)](https://www.abcam.com/ps/products/239/ab239974/Images/ab239974-2-anti-tak1-phospho-s439-antibody-epr2863-immunoprecipitation-hela-human.jpg "Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2863] to TAK1 (phospho S439) - BSA and Azide free
- Suitable for: Dot blot, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free
See all TAK1 primary antibodies -
Description
Rabbit monoclonal [EPR2863] to TAK1 (phospho S439) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
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General notes
ab239974 is the carrier-free version of ab109404.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2863 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
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| Notes |
|---|
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Dot blot
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAssociation with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP. - Information by UniProt
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Database links
- Entrez Gene: 6885 Human
- Entrez Gene: 26409 Mouse
- Entrez Gene: 313121 Rat
- Omim: 602614 Human
- SwissProt: O43318 Human
- SwissProt: Q62073 Mouse
- SwissProt: P0C8E4 Rat
- Unigene: 722892 Human
see all -
Alternative names
- M3K7_HUMAN antibody
- MAP3K 7 antibody
- Map3k7 antibody
see all
Images
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Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
Purified ab109404 at 1/50 dilution (2µg) immunoprecipitating TAK1 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate 10µg
Lane 2 (+): ab109404 + HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab239974 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 75 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109404). -
![Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)](https://www.abcam.com/ps/products/239/ab239974/Images/ab239974-327659-anti-tak1-phospho-s439-antibody-epr2863-dot-blot.jpg)
Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109404).
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![Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)](https://www.abcam.com/ps/products/239/ab239974/Images/ab239974-3-benefits-of-recombinant-antibodies.png)
Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab239974 has not yet been referenced specifically in any publications.
