RecombinantRabMAb

Recombinant Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

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Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
  • Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
  • Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2863] to TAK1 (phospho S439) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free
    See all TAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2863] to TAK1 (phospho S439) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

  • General notes

    ab239974 is the carrier-free version of ab109404.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
Notes
Dot blot
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 67 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC or IHC-P.

Target

  • Function

    Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Association with TAB1/MAP3K7IP1 promotes autophosphorylation and subsequent activation. Association with TAB2/MAP3K7IP2, itself associated with free unanchored Lys-63 polyubiquitin chain, promotes autophosphorylation and subsequent activation of MAP3K7. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.
    Ubiquitinated, leading to proteasomal degradation (By similarity). Requires 'Lys-63'-linked polyubiquitination for autophosphorylation and subsequent activation. 'Lys-63'-linked ubiquitination does not lead to proteasomal degradation. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Deubiquitinated by Y.enterocolitica YopP.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • M3K7_HUMAN antibody
    • MAP3K 7 antibody
    • Map3k7 antibody
    • MEKK7 antibody
    • Mitogen activated protein kinase kinase kinase 7 antibody
    • Mitogen-activated protein kinase kinase kinase 7 antibody
    • TAK1 antibody
    • TGF beta activated kinase 1 antibody
    • TGF-beta-activated kinase 1 antibody
    • TGF1a antibody
    • Transforming growth factor beta activated kinase 1 antibody
    • Transforming growth factor-beta-activated kinase 1 antibody
    see all

Images

  • Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
    Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

    Purified ab109404 at 1/50 dilution (2µg) immunoprecipitating TAK1 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate 10µg
    Lane 2 (+): ab109404 + HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab239974 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 75 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109404).

  • Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
    Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

    Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109404).

  • Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
    Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab239974? Please let us know so that we can cite the reference in this datasheet.

ab239974 has not yet been referenced specifically in any publications.

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