Anti-Tal1 antibody (ab155195)
Key features and details
- Rabbit polyclonal to Tal1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Tal1 antibody -
Description
Rabbit polyclonal to Tal1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 252-331 of Human Tal1 (Uniprot ID: P17542).
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Positive control
- WB: H1299 cell lysate; IHC-P: Mouse spleen tissue, A549 xenograft; IP: HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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| IHC-P | (1) |
1/100 - 1/1000.
Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
| IP |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
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IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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IP
1/100 - 1/500. |
Target
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Function
Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation. -
Tissue specificity
Leukemic stem cell. -
Involvement in disease
Note=A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.
Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6886 Human
- Entrez Gene: 21349 Mouse
- Omim: 187040 Human
- SwissProt: P17542 Human
- SwissProt: P22091 Mouse
- Unigene: 705618 Human
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Alternative names
- bHLHa17 antibody
- Class A basic helix-loop-helix protein 17 antibody
- OTTHUMP00000009563 antibody
see all
Images
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Western blot - Anti-Tal1 antibody - C-terminal (ab155195)Anti-Tal1 antibody (ab155195) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 34 kDa10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody - C-terminal (ab155195)Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue staining Tal1 with ab155195 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunoprecipitation - Anti-Tal1 antibody (ab155195)TAL1 antibody immunoprecipitates TAL1 protein in IP experiments.
IP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
A: HEK-293T whole cell lysate. 40 μg.
B: Control with 2 μg of preimmune rabbit IgG.
C: Immunoprecipitate of TAL1 protein by 2 μg of ab155195.
12% SDS-PAGE
The immunoprecipitated TAL1 protein was detected by ab155195 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody (ab155195)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining Tal1 at cytosol and nucleus with ab155195 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155195 has been referenced in 2 publications.
- Tatsumi G et al. LSD1-mediated repression of GFI1 super-enhancer plays an essential role in erythroleukemia. Leukemia 34:746-758 (2020). PubMed: 31676828
- Zhu Q et al. CUT&RUNTools: a flexible pipeline for CUT&RUN processing and footprint analysis. Genome Biol 20:192 (2019). PubMed: 31500663