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    tal1-antibody-ab155195.pdf

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Cell Biology Cell Cycle Cell differentiation
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Anti-Tal1 antibody (ab155195)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-Tal1 antibody - C-terminal (ab155195)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody - C-terminal (ab155195)
  • Immunoprecipitation - Anti-Tal1 antibody (ab155195)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody (ab155195)

Key features and details

  • Rabbit polyclonal to Tal1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Tal1 antibody
  • Description

    Rabbit polyclonal to Tal1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 252-331 of Human Tal1 (Uniprot ID: P17542).

  • Positive control

    • WB: H1299 cell lysate; IHC-P: Mouse spleen tissue, A549 xenograft; IP: HEK-293T cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Stem Cells
    • Hematopoietic Progenitors
    • Hemangioblast
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P (1)
1/100 - 1/1000.

Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

IP
1/100 - 1/500.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P
1/100 - 1/1000.

Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

IP
1/100 - 1/500.

Target

  • Function

    Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.
  • Tissue specificity

    Leukemic stem cell.
  • Involvement in disease

    Note=A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.
  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.
    Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17542 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6886 Human
    • Entrez Gene: 21349 Mouse
    • Omim: 187040 Human
    • SwissProt: P17542 Human
    • SwissProt: P22091 Mouse
    • Unigene: 705618 Human
    • Alternative names

      • bHLHa17 antibody
      • Class A basic helix-loop-helix protein 17 antibody
      • OTTHUMP00000009563 antibody
      • OTTHUMP00000009564 antibody
      • SCL antibody
      • STEM CELL LEUKEMIA HEMATOPOIETIC TRANSCRIPTION FACTOR antibody
      • Stem cell protein antibody
      • T cell acute lymphocytic leukemia 1 antibody
      • T cell acute lymphocytic leukemia 1 protein antibody
      • T cell acute lymphocytic leukemia 1 protein antibody
      • T cell leukemia/lymphoma 5 protein antibody
      • T-cell acute lymphocytic leukemia protein 1 antibody
      • T-cell leukemia/lymphoma protein 5 antibody
      • Tal 1 antibody
      • Tal 1 product antibody
      • TAL 1 protein antibody
      • TAL bHLH transcription factor 1 erythroid differentiation factor antibody
      • TAL-1 antibody
      • tal1 antibody
      • TAL1_HUMAN antibody
      • TCL 5 antibody
      • TCL5 antibody
      see all

    Images

    • Western blot - Anti-Tal1 antibody - C-terminal (ab155195)
      Western blot - Anti-Tal1 antibody - C-terminal (ab155195)
      Anti-Tal1 antibody (ab155195) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

      Predicted band size: 34 kDa



      10% SDS PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody - C-terminal (ab155195)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody - C-terminal (ab155195)

      Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue staining Tal1 with ab155195 at 1/100 dilution.

      Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

    • Immunoprecipitation - Anti-Tal1 antibody (ab155195)
      Immunoprecipitation - Anti-Tal1 antibody (ab155195)

      TAL1 antibody immunoprecipitates TAL1 protein in IP experiments.

      IP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.

      A: HEK-293T whole cell lysate. 40 μg.

      B: Control with 2 μg of preimmune rabbit IgG.

      C: Immunoprecipitate of TAL1 protein by 2 μg of ab155195.

      12% SDS-PAGE

      The immunoprecipitated TAL1 protein was detected by ab155195 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody (ab155195)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody (ab155195)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining Tal1 at cytosol and nucleus with ab155195 at 1/500 dilution.

      Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab155195? Please let us know so that we can cite the reference in this datasheet.

    ab155195 has been referenced in 2 publications.

    • Tatsumi G  et al. LSD1-mediated repression of GFI1 super-enhancer plays an essential role in erythroleukemia. Leukemia 34:746-758 (2020). PubMed: 31676828
    • Zhu Q  et al. CUT&RUNTools: a flexible pipeline for CUT&RUN processing and footprint analysis. Genome Biol 20:192 (2019). PubMed: 31500663

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tal1 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH8, 100C, 20min
    Sample
    Human Tissue sections (intestine)
    Specification
    intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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