Key features and details
- Mouse monoclonal [OTI6A2] to Tal1
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Rat, Dog, Human
- Isotype: IgG1
Product nameAnti-Tal1 antibody [OTI6A2]
See all Tal1 primary antibodies
DescriptionMouse monoclonal [OTI6A2] to Tal1
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WBmore details
Species reactivityReacts with: Rat, Dog, Human
Predicted to work with: Mouse
Recombinant full length protein corresponding to Human Tal1 aa 1-331. Produced in HEK-293T cells (NP_003180).
MTERPPSEAARSDPQLEGRDAAEASMAPPHLVLLNGVAKETSRAAAAEPP VIELGARGGPGGGPAGGGGAARDLKGRDAATAEARHRVPTTELCRPPGPA PAPAPASVTAELPGDGRMVQLSPPALAAPAAPGRALLYSLSQPLASLGSG FFGEPDAFPMFTTNNRVKRRPSPYEMEITDGPHTKVVRRIFTNSRERWRQ QNVNGAFAELRKLIPTHPPDKKLSKNEILRLAMKYINFLAKLLNDQEEEG TQRAKTGKDPVVGAGGGGGGGGGGAPPDDLLQDVLSPNSSCGSSLDGAAS PDSYTEEPAPKHTARSLHPAMLPAADGAGPR
Database link: P17542
- WB: pCMV6-ENTRY Tal1 cDNA transfected HEK-293T cell lysate; MDCK and PC-12 cell lysates. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY Tal1 cDNA. Flow: HEK-293T cells.
Clone OTI6A2 (formerly 6A2).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 34 kDa.|
FunctionImplicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.
Tissue specificityLeukemic stem cell.
Involvement in diseaseNote=A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
DomainThe helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.
modificationsPhosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.
Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.
- Information by UniProt
- bHLHa17 antibody
- Class A basic helix-loop-helix protein 17 antibody
- OTTHUMP00000009563 antibody
All lanes : Anti-Tal1 antibody [OTI6A2] (ab236520) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY Tal1 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 34 kDa
All lanes : Anti-Tal1 antibody [OTI6A2] (ab236520) at 1/200 dilution
Lane 1 : MDCK (Canine kidney cell line) cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY Tal1 cDNA stained for Tal1 (green) using ab236520 at 1/100 dilution in ICC/IF.
Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling Tal1 with ab236520 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236520 has been referenced in 1 publication.
- Godavarthy PS et al. The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis. Haematologica 105:136-147 (2020). PubMed: 31018977