Product nameAnti-Talin 1 and 2 antibody [8D4]
See all Talin 1 and 2 primary antibodies
DescriptionMouse monoclonal [8D4] to Talin 1 and 2
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, ICC, IPmore details
Species reactivityReacts with: Rat, Human, Xenopus laevis
Does not react with: Fish
Full length native protein (purified) corresponding to Chicken Talin 1 and 2.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR228677. Lot numbers higher than GR228677 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue culture supernatant.
Our Abpromise guarantee covers the use of ab11188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100. See Abreview.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 225 kDa.
Use at an assay dependent concentration.
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
RelevanceTalin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.
Cellular localizationCell projection, ruffle membrane (talin 1), Focal adhesion plaques and synapses (talin 2)
- talin 1 antibody
- talin 2 antibody
- talin antibody
Central Panel: ab11188 staining Talin 1 and 2 in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
Left-hand panel: Nuclei counterstained with DAPI (blue).
Right-hand panel: Overlay
ab11188 staining Talin 1 and 2 in Human platelet cells by Flow cytometry.P : Permeabilized;
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated goat anti-mouse IgG (H+L) at a 1/500 dilution.
US : Unstained, Red Peak; IGG MS : IgG Mouse (Isotype Control), Blue Peak;
Talin, Green peak.
This product has been referenced in:
- Chang TY et al. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. Elife 6:N/A (2017). Read more (PubMed: 29271742) »
- Liu YJ et al. Confinement and low adhesion induce fast amoeboid migration of slow mesenchymal cells. Cell 160:659-672 (2015). WB . Read more (PubMed: 25679760) »