Key features and details
- Mouse monoclonal [8D4] to Talin 1 and 2
- Reacts with: Rat, Human, Xenopus laevis
- Isotype: IgG1
Product nameAnti-Talin 1 and 2 antibody [8D4]
See all Talin 1 and 2 primary antibodies
DescriptionMouse monoclonal [8D4] to Talin 1 and 2
Species reactivityReacts with: Rat, Human, Xenopus laevis
Does not react with: Fish
Full length native protein (purified) corresponding to Chicken Talin 1 and 2.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR228677. Lot numbers higher than GR228677 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
Purification notesPurified from Tissue culture supernatant.
RelevanceTalin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.
Cellular localizationCell projection, ruffle membrane (talin 1), Focal adhesion plaques and synapses (talin 2)
- talin 1 antibody
- talin 2 antibody
- talin antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab11188 has been referenced in 13 publications.
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- Saleh A et al. A novel 3D nanofibre scaffold conserves the plasticity of glioblastoma stem cell invasion by regulating galectin-3 and integrin-ß1 expression. Sci Rep 9:14612 (2019). PubMed: 31601895
- Chapelle J et al. Dissecting the Shared and Context-Dependent Pathways Mediated by the p140Cap Adaptor Protein in Cancer and in Neurons. Front Cell Dev Biol 7:222 (2019). PubMed: 31681758
- Chang TY et al. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. Elife 6:N/A (2017). PubMed: 29271742
- Liu YJ et al. Confinement and low adhesion induce fast amoeboid migration of slow mesenchymal cells. Cell 160:659-672 (2015). WB . PubMed: 25679760