Product nameAnti-Talin 1 antibody [8D4]
See all Talin 1 primary antibodies
DescriptionMouse monoclonal [8D4] to Talin 1
Tested applicationsSuitable for: WB, IP, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Frog
Talin 1 protein purified from chicken gizzard.
Epitopeab157808 recognizes an epitope within the rod domain of Talin 1.
- A431, PC12 and Rabbit fibroblast cell lysates; Chick fibroblasts
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 48% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab157808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 269 kDa).|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
Sequence similaritiesContains 1 FERM domain.
Contains 1 I/LWEQ domain.
Cellular localizationCell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
- Information by UniProt
- ILWEQ antibody
- Talin 1 antibody
- Talin antibody
Lanes 1-2 : Anti-Talin 1 antibody [8D4] (ab157808) at 1/1000 dilution
Lanes 3-4 : Anti-Talin 1 (phospho S425) antibody (ab157807) at 1/1000 dilution (Rabbit polyclonal to anti-Talin 1 (phospho Serine 425))
Lanes 1 & 3 : PC12 cell lysate - stimulated with calyculin A at 100nM for 30 minutes
Lanes 2 & 4 : PC12 cell lysate - stimulated with calyculin A at 100nM for 30 minutes and treated with lambda phosphatase
Predicted band size: 269 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS and 0.04% Tween20 for 1 hour at room temperature.
Overlay histogram showing RAW 264.7 cells stained with ab157808 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157808, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunocytochemical analysis of chick fibroblasts labelling Talin 1 with ab157808 at 1/100 (red). F-actin was stained in green.
Image provided by Dr. Gianluca Gallo at Drexel University.
This product has been referenced in:
- Li X et al. Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway. EBioMedicine N/A:N/A (2018). Read more (PubMed: 30545799) »
- Röselová P et al. Adhesion structures in leukemia cells and their regulation by Src family kinases. Cell Adh Migr 12:286-298 (2018). Read more (PubMed: 28678601) »