Overview

  • Product name
    Anti-Talin 1 antibody [8D4]
    See all Talin 1 primary antibodies
  • Description
    Mouse monoclonal [8D4] to Talin 1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Frog
  • Immunogen

    Talin 1 protein purified from chicken gizzard.

  • Epitope
    ab157808 recognizes an epitope within the rod domain of Talin 1.
  • Positive control
    • A431, PC12 and Rabbit fibroblast cell lysates; Chick fibroblasts

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 48% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    8D4
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 269 kDa).
IP 1/100.
ICC/IF 1/100.
Flow Cyt 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Overlay histogram showing RAW 264.7 cells stained with ab157808 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157808, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Lanes 1-2 : Anti-Talin 1 antibody [8D4] (ab157808) at 1/1000 dilution
    Lanes 3-4 : Anti-Talin 1 (phospho S425) antibody (ab157807) at 1/1000 dilution (Rabbit polyclonal to anti-Talin 1 (phospho Serine 425))

    Lanes 1 & 3 : PC12 cell lysate - stimulated with calyculin A at 100nM for 30 minutes
    Lanes 2 & 4 : PC12 cell lysate - stimulated with calyculin A at 100nM for 30 minutes and treated with lambda phosphatase

    Predicted band size: 269 kDa
    Observed band size: 240 kDa
    why is the actual band size different from the predicted?



    Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS and 0.04% Tween20 for 1 hour at room temperature.
  • Immunocytochemical analysis of chick fibroblasts labelling Talin 1 with ab157808 at 1/100 (red). F-actin was stained in green.
    Image provided by Dr. Gianluca Gallo at Drexel University.

References

This product has been referenced in:
  • Yuan D  et al. Effect of integrin-linked kinase gene silencing on microRNA expression in ovarian cancer. Mol Med Rep 16:7267-7276 (2017). Read more (PubMed: 28944870) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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