Flow Cytometry abreview for Anti-Talin 1 antibody

Excellent
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (platelet)
Specification
platelet
Preparation
Cell harvesting/tissue preparation method: fixed, washed platelets from whole blood
Sample buffer: walsh buffer
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% saponin
Gating Strategy
settings for platelet population, ungated

Other product details

Concentration
3.3 µg/ml
Incubation time
20 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Dye-light anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 649
Dilution
1/200

Additional data

Additional Notes
We used Abc71333 to look for intracellular talin (shown in green) in fixed, permeabilized platelets. It had a very good signal to noise ration (cf IgG, shown in red)and worked at a fairly low concentration.

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Submitted Sep 21 2012

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