Western blot abreview for Anti-Talin 1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
20 µg
Specification
platelet
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS-T, 2% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Odyssey goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Licor
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 280 kDa

Additional data

Additional Notes
Ab 71333 against talin, gives a very strong and clean band both for mouse and human whole cell lysate. Strongly recommended.

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Submitted Nov 08 2012

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