ab157807 detects a 240 kDa protein corresponding to the molecular mass of Talin 1 on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment. The immunogen sequence is conserved in Talin 2 (Ser-428).
Rat, Rabbit, Chicken, Human Predicted to work with:
Synthetic peptide (Human) coupled to a carrier protein, corresponding to amino acids surrounding phospho-Serine 425 in Human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 269 kDa).
Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
Contains 1 FERM domain. Contains 1 I/LWEQ domain.
Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell surface. Cell junction, focal adhesion. Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity).
Western blot - Anti-Talin 1 (phospho S425) antibody (ab157807)
Lanes 1-2 : Mouse monoclonal to Talin 1 at 1/1000 dilution Lanes 3-4 : Anti-Talin 1 (phospho S425) antibody (ab157807) at 1/1000 dilution
Lanes 1 & 3 : Rat PC12 cell lysate - stimulated with Calyculin A for 30 minutes at 100nM Lanes 2 & 4 : Rat PC12 cell lysate - stimulated with Calyculin A for 30 minutes at 100nM and treated with lambda phosphatase
Predicted band size: 269 kDa
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS and 0.04% Tween 20 for 1 hour at room temperature.
Immunocytochemical analysis of chick fibroblasts labelling Talin 1 phosphorylated at Serine 425 with ab157807 at 1/200 (green). F-actin stained in red.
(Image provided by Dr. Gianluca Gallo at Drexel University).