Anti-Talin 1 (phospho S425) antibody (ab157807)

Overview

  • Product name
    Anti-Talin 1 (phospho S425) antibody
    See all Talin 1 primary antibodies
  • Description
    Rabbit polyclonal to Talin 1 (phospho S425)
  • Host species
    Rabbit
  • Specificity
    ab157807 detects a 240 kDa protein corresponding to the molecular mass of Talin 1 on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
    The immunogen sequence is conserved in Talin 2 (Ser-428).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Chicken, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) coupled to a carrier protein, corresponding to amino acids surrounding phospho-Serine 425 in Human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).

  • Positive control
    • PC12, A431, Rabbit fibroblast cell lysate treated with Calyculin A; Chick fibroblasts

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 269 kDa).
ICC/IF 1/200.

Target

Images

  • Lanes 1-2 : Mouse monoclonal to Talin 1 at 1/1000 dilution
    Lanes 3-4 : Anti-Talin 1 (phospho S425) antibody (ab157807) at 1/1000 dilution

    Lanes 1 & 3 : Rat PC12 cell lysate - stimulated with Calyculin A for 30 minutes at 100nM
    Lanes 2 & 4 : Rat PC12 cell lysate - stimulated with Calyculin A for 30 minutes at 100nM and treated with lambda phosphatase

    Predicted band size: 269 kDa



    Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS and 0.04% Tween 20 for 1 hour at room temperature.
  • Immunocytochemical analysis of chick fibroblasts labelling Talin 1 phosphorylated at Serine 425 with ab157807 at 1/200 (green). F-actin stained in red.
    (Image provided by Dr. Gianluca Gallo at Drexel University).

References

ab157807 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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