Anti-Talin 2 antibody [68E7] (ab105458)
Key features and details
- Mouse monoclonal [68E7] to Talin 2
- Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IP
- Reacts with: Mouse
- Isotype: IgG2b
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Talin 2 antibody [68E7]
See all Talin 2 primary antibodies -
Description
Mouse monoclonal [68E7] to Talin 2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment, corresponding to amino acids 489-911 of Mouse Talin 2.
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Positive control
- Fibroblasts
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General notes
Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37°C + 5%CO2The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
68E7 -
Myeloma
NS0/1 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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|
ICC/IF |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
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|
IP |
Use at an assay dependent concentration.
|
Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Target
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Function
As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity. -
Sequence similarities
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
Cellular localization
Cell junction > focal adhesion. Cell junction > synapse. Cell membrane. Cytoplasm > cytoskeleton. Focal adhesion plaques and synapses. - Information by UniProt
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Database links
- Entrez Gene: 70549 Mouse
- SwissProt: Q71LX4 Mouse
- Unigene: 33645 Mouse
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Alternative names
- DKFZp451B1011 antibody
- DKFZp686I0976 antibody
- DKFZp686K0979 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab105458 has been referenced in 9 publications.
- Hadjisavva R et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition. Cell Rep 40:111091 (2022). PubMed: 35858563
- Han SJ et al. Pre-complexation of talin and vinculin without tension is required for efficient nascent adhesion maturation. Elife 10:N/A (2021). PubMed: 33783351
- Kanoldt V et al. Metavinculin modulates force transduction in cell adhesion sites. Nat Commun 11:6403 (2020). PubMed: 33335089
- Böttcher RT et al. Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading. J Cell Biol 216:3785-3798 (2017). PubMed: 28912124
- Theodosiou M et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. Elife 5:e10130 (2016). WB ; Mouse . PubMed: 26821125
- Bouchet BP et al. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. Elife 5:N/A (2016). WB . PubMed: 27410476
- Fang KP et al. Both Talin-1 and Talin-2 correlate with malignancy potential of the human hepatocellular carcinoma MHCC-97 L cell. BMC Cancer 16:45 (2016). PubMed: 26822056
- Austen K et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol 17:1597-606 (2015). PubMed: 26523364
- Debrand E et al. Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms. FEBS J 276:1610-28 (2009). PubMed: 19220457