Recombinant Anti-Talin 2 antibody [EPR2536(2)] (ab108967)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2536(2)] to Talin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-Talin 2 antibody [EPR2536(2)]
See all Talin 2 primary antibodies -
Description
Rabbit monoclonal [EPR2536(2)] to Talin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- COS-1, 293T, HeLa, and Jurkat whole cell lysate (ab7899)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2536(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa). |
Target
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Function
As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity. -
Sequence similarities
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
Cellular localization
Cell junction > focal adhesion. Cell junction > synapse. Cell membrane. Cytoplasm > cytoskeleton. Focal adhesion plaques and synapses. - Information by UniProt
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Database links
- Entrez Gene: 83660 Human
- Entrez Gene: 70549 Mouse
- Entrez Gene: 315776 Rat
- Omim: 607349 Human
- SwissProt: Q9Y4G6 Human
- SwissProt: Q71LX4 Mouse
- Unigene: 569438 Human
- Unigene: 610975 Human
see all -
Alternative names
- DKFZp451B1011 antibody
- DKFZp686I0976 antibody
- DKFZp686K0979 antibody
see all
Images
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All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + Jurkat cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + COS-1 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (unpurified)
Lane 1 : COS-1 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab108967 has been referenced in 3 publications.
- Wu Y et al. Long Noncoding RNA Lnc-TLN2-4:1 Suppresses Gastric Cancer Metastasis and Is Associated with Patient Survival. J Oncol 2020:8681361 (2020). PubMed: 32256587
- van de Klundert MA et al. Hepatitis B Virus Protein X Induces Degradation of Talin-1. Viruses 8:N/A (2016). WB ; Human . PubMed: 27775586
- Formosa A et al. DNA methylation silences miR-132 in prostate cancer. Oncogene : (2012). PubMed: 22310291