Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab171120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 10 µg/mg of lysate.|
- 2-[3,6-bis(dimethylamino)xanthenium-9-yl]benzoate antibody
- 2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate antibody
- CHEBI:52282 antibody
Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 µg protein with 10 µg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.
Lane 1: ab171120
Lane 2: BSA
Lane 3: TAMRA-BSA
Lane 4: BSA-ab171120
Lane 5: TAMRA-BSA + mouse IgG
Lane 6: TAMRA-BSA + ab171120
All lanes : Anti-TAMRA antibody [5G5] (ab171120) at 1/1000 dilution
Lane 1 : TAMRA-BSA at 10 µg
Lane 2 : TAMRA-BSA at 5 µg
Lane 3 : TAMRA-BSA at 2 µg
Lane 4 : TAMRA-BSA at 1 µg
Lane 5 : TAMRA-BSA at 0.5 µg
Lane 6 : TAMRA-BSA at 0 µg
All lanes : Goat anti-mouse-HRP
Developed using the ECL technique.
ab171120 has not yet been referenced specifically in any publications.