Key features and details
- Mouse monoclonal [5G5] to TAMRA
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-TAMRA antibody [5G5]
See all TAMRA primary antibodies
DescriptionMouse monoclonal [5G5] to TAMRA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to TAMRA.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab171120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 10 µg/mg of lysate.|
- 2-[3,6-bis(dimethylamino)xanthenium-9-yl]benzoate antibody
- 2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate antibody
- CHEBI:52282 antibody
Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 µg protein with 10 µg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.
Lane 1: ab171120
Lane 2: BSA
Lane 3: TAMRA-BSA
Lane 4: BSA-ab171120
Lane 5: TAMRA-BSA + mouse IgG
Lane 6: TAMRA-BSA + ab171120
All lanes : Anti-TAMRA antibody [5G5] (ab171120) at 1/1000 dilution
Lane 1 : TAMRA-BSA at 10 µg
Lane 2 : TAMRA-BSA at 5 µg
Lane 3 : TAMRA-BSA at 2 µg
Lane 4 : TAMRA-BSA at 1 µg
Lane 5 : TAMRA-BSA at 0.5 µg
Lane 6 : TAMRA-BSA at 0 µg
All lanes : Goat anti-mouse-HRP
Developed using the ECL technique.
ab171120 has not yet been referenced specifically in any publications.